Genes required for ionizing radiation resistance in yeast
Genes required for ionizing radiation resistance in yeast
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DOI：
10.1038/ng778
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发表时间：
2001-12-01
期刊：
影响因子：
30.8
通讯作者：
Resnick, MA
中科院分区：
文献类型：
--
作者：
Bennett, CB;Lewis, LK;Resnick, MA
酿酒酵母耐受电离辐射损伤的能力需要许多DNA修复和检查点基因，其中大多数具有人类直系同源物。对3,670个非必需基因缺失纯合的二倍体突变体进行全基因组筛选，发现了107个影响γ射线敏感性的新基因座。许多影响复制，重组和检查点功能。近90%的新基因对其他药物敏感，大多数新基因可以被分配到以下功能组：染色质重塑，染色体分离，核孔形成，转录，高尔基体/液泡活动，泛素介导的蛋白质降解，胞质分裂，线粒体活性和细胞壁维护。超过50%的基因与人类基因具有同源性，包括17个与癌症有关的基因，这表明大量新发现的人类基因可能在耐受辐射损伤方面发挥相关作用。
The ability of Saccharomyces cerevisiae to tolerate ionizing radiation damage requires many DNA-repair and checkpoint genes, most having human orthologs. A genome-wide screen of diploid mutants homozygous with respect to deletions of 3,670 nonessential genes revealed 107 new loci that influence gamma -ray sensitivity. Many affect replication, recombination and checkpoint functions. Nearly 90% were sensitive to other agents, and most new genes could be assigned to the following functional groups: chromatin remodeling, chromosome segregation, nuclear pore formation, transcription, Golgi/vacuolar activities, ubiquitin-mediated protein degradation, cytokinesis, mitochondrial activity and cell wall maintenance. Over 50% share homology with human genes, including 17 implicated in cancer, indicating that a large set of newly identified human genes may have related roles in the toleration of radiation damage.