Immune system and prognosis in colorectal cancer: a detailed immunohistochemical analysis
Immune system and prognosis in colorectal cancer: a detailed immunohistochemical analysis
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DOI：
10.1038/labinvest.3700055
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发表时间：
2004-04-01
影响因子：
5
通讯作者：
Kuppen, PJK
中科院分区：
文献类型：
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作者：
Menon, AG;Janssen, CM;Kuppen, PJK
肿瘤细胞-免疫细胞相互作用的功效取决于许多因素，例如，肿瘤细胞上HLA-I的表达、免疫细胞的类型、肿瘤细胞对免疫细胞的可及性和免疫原性表位的表达。我们评估了93例结直肠癌的肿瘤上皮、肿瘤间质和肿瘤边缘中CD4（+）、CD8（+）、CD56（+）和CD57（+）细胞的浸润，并将其与临床病理参数、肿瘤细胞上HLA-A和HLA-B/C的表达、肿瘤结节周围存在基底膜（BM）样结构和存在微卫星不稳定性/突变子表型（不存在MLH-1表达）。上皮内CD4（+）、CD8（+）、CD56（+）和CD57（+）细胞浸润的中位数分别为3、23、0和0个细胞/mm2肿瘤。肿瘤细胞HLA-A/BC表达正常者占28/43%，异质性表达者占59/48%，缺失者占13/9%。47%、38%和15%的病例肿瘤结节周围BM样结构缺失、存在和增厚。6例MLH1表达缺失。CD4（+）、CD8（+）、CD56（+）、CD57（+）三个亚群的白细胞浸润与淋巴结转移呈正相关（均P
The efficacy of tumor cell-immune cell interactions depends on a number of factors, for example, the expression of HLA-I on tumor cells, the type of immune cell, the accessibility of tumor cells for immune cells and the expression of immunogenic epitopes. We assessed infiltration of CD4(+), CD8(+), CD56(+) and CD57(+) cells in the tumor epithelium, tumor stroma and advancing tumor margin of 93 colorectal carcinomas and correlated this to clinicopathological parameters, the expression of HLA-A and HLA-B/C on tumor cells, the presence of a basal membrane (BM)-like structure surrounding tumor nodules and the presence of microsatellite instability/ mutator phenotype (absent MLH-1 expression). The median intraepithelial CD4(+), CD8(+), CD56(+) and CD57(+) cell infiltrations were 3, 23, 0 and 0 cells/mm(2) tumor, respectively. HLA-A/BC expression by tumor cells was normal in 28/43%, heterogeneous in 59/48% and absent in 13/9% of the cases. A BM-like structure surrounding the tumor nodules was absent, present and thick in 47, 38 and 15% of the cases. Six cases lost MLH1 expression. There was a positive correlation between leukocyte infiltration in the three compartments for CD4(+), CD8(+), CD56(+) (partly) and CD57(+) (all P