IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation
IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation
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IRW 和 IQW 通过减轻 DSS 引起的结肠炎症来降低结肠炎相关癌症风险
DOI：
10.1155/2019/6429845
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发表时间：
2019-10-24
影响因子：
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通讯作者：
Liu, Gang
中科院分区：
文献类型：
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作者：
Ma, Yong;Jiang, Hongmei;Liu, Gang
背景和目的。生物活性肽通过靶向作用于胃肠道而对动物和人类健康产生重要影响。采用葡聚糖硫酸钠（DSS）诱导小鼠结肠炎模型。将32只平均体重23 g的8周龄小鼠随机分为4组，每组8只：饲喂基础饮食（CON）的小鼠、饲喂基础饮食+5% DSS（DSS）的小鼠、饲喂0.03% IRW +5% DSS（IRW-DSS）的小鼠和饲喂0.03% IRW +5% DSS（IQW-DSS）的小鼠。在3天的适应期后，在第8天，屠宰所有小鼠。收集血清样品以测定氨基酸水平;快速冷冻结肠组织以测定基因表达。方法.本研究的目的是根据血清氨基酸和肠道炎症因子来评估两种肽（IRW和IQW）在DSS诱导的模型中修复肠道炎症的能力。结果结果表明，IRW和IQW的添加对DSS诱导的肠道炎症具有减轻作用。补充IRW-DSS的小鼠血清中Asp水平降低（P
Background and Objective. Bioactive peptides exert great influence in animals and human health by targeting gastrointestinal tracts. The colitis model of mice was induced by dextran sulfate sodium (DSS). Thirty-two 8-week-old mice weighing 23 g on average were randomly assigned to four groups of 8 each: mice fed basal diet (CON), mice fed basal diet with 5% DSS (DSS), mice fed 0.03% IRW with 5% DSS (IRW-DSS), and mice fed 0.03% IRW with 5% DSS (IQW-DSS). After an adaptation period of 3 days, on day 8, all mice were slaughtered. Serum samples were collected to determine the level of amino acids; colonic tissue was quick-frozen for the determination of gene expression. Methods. The aim of this study was to assess the ability of two kinds of peptides (IRW and IQW) to repair intestinal inflammatory in the DSS-induced model in accordance with serum amino acids and intestinal inflammatory factors. Results. The results demonstrated that the addition of IRW and IQW had a mitigating effect on DSS-induced intestinal inflammation. The level of Asp decreased in the serum of mice supplemented with IRW-DSS (P