PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.
PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.
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DOI：
10.1016/j.febslet.2014.05.038
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发表时间：
2014-06-27
期刊：
影响因子：
3.5
通讯作者：
Baker A
中科院分区：
文献类型：
--
作者：
Lanyon-Hogg T;Hooper J;Gunn S;Warriner SL;Baker A
拟南芥PEX 5和PEX 14 N之间的相互作用不依赖于货物结合。PTS 1肽对PEX 5的亲和力不受PEX 14 N结合的影响。拟南芥PEX 5复合物PTS 1和PTS 2货物。PEX 5和7与PEX 14 N共分离，但PTS 2货物硫解酶不共分离。PEX 14 N在体外不会卸载典型的PTS 1货物肽，但可能在PTS 2释放中发挥作用。PEX 5作为循环受体将PTS 1蛋白导入过氧化物酶体，并作为PEX 7的共受体，PTS 2受体，但货物卸载的机制仍然不清楚。使用重组蛋白结构域，我们显示PEX 5与PEX 14 N-末端结构域（PEX 14 N）的结合对PEX 5对含有PTS 1的肽的亲和力没有影响。PEX 5可以形成同时含有重组PTS 1货物和内源性PEX 7-硫解酶的复合物，但是通过PEX 14构建体分离复合物导致硫解酶的缺失，这表明PEX 14在PTS 2货物的卸载中可能起作用。pMDH 1通过下拉与PEX 5物理相互作用（查看相互作用）PEX 5C通过过滤器结合与PEX 14 N结合（查看互动）PEX 14 N通过下拉与PEX 5C结合（查看交互）PEX 14 N通过下拉与PEX 7进行物理交互（查看交互）PEX 5通过下拉与PEX 7进行物理交互（查看交互）DCI 1通过下拉与PEX 5进行物理交互（查看相互作用）PEX 5通过下拉与硫解酶PTS 2-货物物理相互作用（查看相互作用）pMDH 1通过下拉与PEX 7发生物理相互作用（查看相互作用）DCI 1通过下拉与硫解酶PTS 2-货物发生物理相互作用（查看交互）DCI 1通过下拉与PEX 7进行物理交互（查看交互）PEX 14 N通过下拉与PEX 5进行物理交互（查看交互）
The interaction between Arabidopsis PEX5 and PEX14N is independent of cargo binding. The affinity of a PTS1 peptide for PEX5 is unaffected by PEX14N binding. Arabidopsis PEX5 complexes PTS1 and PTS2 cargoes. PEX5 and 7 co-isolate with PEX14N, but the PTS2 cargo thiolase does not. PEX14N does not unload canonical PTS1 cargo peptide in vitro but may play a role in PTS2 release. PEX5 acts as a cycling receptor for import of PTS1 proteins into peroxisomes and as a co-receptor for PEX7, the PTS2 receptor, but the mechanism of cargo unloading has remained obscure. Using recombinant protein domains we show PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptide. PEX5 can form a complex containing both recombinant PTS1 cargo and endogenous PEX7-thiolase simultaneously but isolation of the complex via the PEX14 construct resulted in an absence of thiolase, suggesting a possible role for PEX14 in the unloading of PTS2 cargos. pMDH1physically interacts with PEX5 by pull down (View interaction) PEX5Cbinds to PEX14N by filter binding (View interaction) PEX14Nbinds to PEX5C by pull down (View interaction) PEX14Nphysically interacts with PEX7 by pull down (View interaction) PEX5physically interacts with PEX7 by pull down (View interaction) DCI1physically interacts with PEX5 by pull down (View interaction) PEX5physically interacts with thiolase PTS2-cargo by pull down (View interaction) pMDH1physically interacts with PEX7 by pull down (View interaction) DCI1physically interacts with thiolase PTS2-cargo by pull down (View interaction) DCI1physically interacts with PEX7 by pull down (View interaction) PEX14Nphysically interacts with PEX5 by pull down (View interaction)
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影响因子：
3.7
作者：
Mitsuya S;El-Shami M;Sparkes IA;Charlton WL;Lousa Cde M;Johnson B;Baker A
通讯作者：
Baker A
影响因子：
4.9
作者：
Nito, K;Hayashi, M;Nishimura, M
通讯作者：
Nishimura, M
影响因子：
4.8
作者：
Hayashi, M;Yagi, M;Nishimura, M
通讯作者：
Nishimura, M
影响因子：
4.8
作者：
Alencastre, Ines S.;Rodrigues, Tony A.;Azevedo, Jorge E.
通讯作者：
Azevedo, Jorge E.
影响因子：
4.1
作者：
Harano, T;Nose, S;Fujiki, Y
通讯作者：
Fujiki, Y