Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function
Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function
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DOI：
10.1016/s0925-4773(99)00316-0
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发表时间：
2000-03-01
影响因子：
2.6
通讯作者：
Sun, YH
中科院分区：
文献类型：
--
作者：
Jaw, TJ;You, LR;Sun, YH
果蝇同型胸（HTH）和外腹（EXD）是许多发育过程中需要的两种同型蛋白。EXD可以作为Hox蛋白的辅助因子。它的核定位依赖于HTH。在这项研究中，我们提出了HTH和EXD之间的体内物理相互作用的证据，主要通过HTH中进化保守的MH结构域介导，这种相互作用对于两种蛋白质的相互稳定，EXD核定位以及EXD-HTH异源二聚体的DNA合作结合至关重要。一些体内功能需要细胞核中的EXD和HTH，这表明EXD-HTH复合物可能作为转录调节剂起作用。(C) 2000爱思唯尔科学爱尔兰有限公司版权所有。
The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH, This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.