Dysregulation of chemokine receptor expression and function by B cells of patients with primary Sjogren's syndrome
Dysregulation of chemokine receptor expression and function by B cells of patients with primary Sjogren's syndrome
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DOI：
10.1002/art.21129
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Dörner, T
中科院分区：
文献类型：
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作者：
Hansen, A;Reiter, K;Dörner, T
Objective.评估趋化因子受体表达异常和/或对同源配体的反应异常是否可能是原发性干燥综合征（SS）B细胞稳态特征性紊乱的基础。采用流式细胞术、单细胞逆转录-聚合酶链反应（RT-PCR）和迁移试验分析了原发性SS患者和健康对照者的CD 27- naive和CD 27 + -memory B细胞的趋化因子受体表达。与健康受试者相比，原发性SS患者外周血B细胞表面CXCR 4和CXCR 4信使RNA（mRNA）的表达显著升高。这些差异在CD 27初始B细胞中最为显著（P
Objective. To assess whether abnormal chemokine receptor expression and/or abnormal responsiveness to the cognate ligands might underlie some of the disturbances in B cell homeostasis characteristic of primary Sjogren's syndrome (SS).Methods. Chemokine receptor expression by CD27- naive and CD27+ -memory B cells from patients with primary SS and healthy control subjects was analyzed using flow cytometry, single-cell reverse transcriptase-polymerase chain reaction (RT-PCR), and migration assays.Results. In contrast to healthy subjects, significantly higher expression of both surface CXCR4 and CXCR4 messenger RNA (mRNA) was seen in peripheral blood B cells from patients with primary SS. These differences were most prominent in CD27- naive B cells (P