Rapid inhibition of c-myc gene expression by a glucocorticoid in the avian oviduct.
Rapid inhibition of c-myc gene expression by a glucocorticoid in the avian oviduct.
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DOI：
10.1210/mend-3-6-991
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发表时间：
1989-06
影响因子：
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通讯作者：
C. Rories;C. Lau;K. Fink;T. Spelsberg
中科院分区：
文献类型：
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作者：
C. Rories;C. Lau;K. Fink;T. Spelsberg
糖皮质激素地塞米松 (DEX) 会导致经雌激素处理的未成熟鸡的输卵管中 c-myc mRNA 水平快速、可逆地降低。注射 0.5 mg DEX 后，c-myc mRNA 水平开始下降 5 分钟，30 分钟时最低达到对照值的 10%，注射后 4 小时恢复至对照值的 30-40%。 DEX 对 c-myc mRNA 水平的这种快速影响发生在己烯雌酚刺激和己烯雌酚撤回的输卵管中。该效果具有剂量依赖性，仅注射 10 微克 DEX（0.03 微克/克体重）即可测量 c-myc mRNA 的减少。类固醇的作用是基因特异性的，H2B 组蛋白 mRNA 显示出显着降低的反应。该效果也是组织特异性的，注射 0.5 mg DEX 后 30 分钟，肝脏的 c-myc mRNA 水平比对照值增加了 170%。 DEX 降低禽类输卵管 c-myc mRNA 水平可能在糖皮质激素抑制该组织中细胞增殖的过程中发挥作用。类固醇对c-myc表达的快速作用使得类固醇诱导的c-myc mRNA水平降低很可能代表类固醇-受体复合物对c-myc基因表达的直接主要作用。
The glucocorticoid dexamethasone (DEX) causes a rapid, reversible reduction in c-myc mRNA level in the oviducts of estrogen-treated, immature chickens. The c-myc mRNA level begins to decrease by 5 min after injection of 0.5 mg DEX, reaches a minimum of 10% of the control value by 30 min, and returns to 30-40% of the control value by 4 h post injection. This rapid effect of DEX on the c-myc mRNA level occurs in both diethylstilbestrol-stimulated and diethylstilbestrol-withdrawn oviducts. The effect is dose dependent, with reduction of the c-myc mRNA measured with as little as 10 micrograms DEX injection (0.03 micrograms/g BW). The effect of the steroid is gene specific with H2B histone mRNA displaying a significantly reduced response. The effect is also tissue specific with liver displaying an increase of 170% of control values in c-myc mRNA level by 30 min after injection of 0.5 mg DEX. The reduction of avian oviduct c-myc mRNA levels by DEX may play a role in glucocorticoid inhibition of cell proliferation in this tissue. The rapidity of the steroid effects on c-myc expression makes it likely that the steroid-induced reduction of c-myc mRNA levels represents a direct primary action of the steroid-receptor complex on the c-myc gene expression.