Isolation and characterization of IS 31831, a transposable element from Corynebacterium glutamicum
Isolation and characterization of IS 31831, a transposable element from Corynebacterium glutamicum
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IS 31831（谷氨酸棒杆菌转座元件）的分离和表征
DOI：
10.1111/j.1365-2958.1994.tb00351.x
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发表时间：
1994
影响因子：
3.6
通讯作者：
H. Yukawa
中科院分区：
文献类型：
--
作者：
A. Vertès;M. Inui;Miki Kobayashi;Y. Kurusu;H. Yukawa
从棒状细菌谷氨酸棒杆菌ATCC 31831中分离并鉴定了转座因子。IS 31831是一个1453 bp的插入序列，含有24 bp的不完全末端反向重复序列.它含有一个开放阅读框，在氨基酸水平上与来自耻垢分枝杆菌的IS 1096转座酶高度同源。IS 31831和IS 1096都表现出几个共同的特征，表明它们构成了一个新的插入序列家族。IS 31831是利用枯草芽孢杆菌sacB基因表达所赋予的棒状细菌的蔗糖敏感性而分离的。构建了用于从棒状杆菌属细菌中分离转座因子的大肠杆菌/棒状杆菌穿梭载体。
A transposable element from a coryneform bacterium, Corynebacterium glutamicum ATCC 31831 was isolated and characterized. The element IS 31831 is a 1453 bp insertion sequence with 24 bp imperfect terminal inverted repeats. It contains one open reading frame highly homologous at the amino acid level to the transposase of IS 1096 from Mycobacterium smeg‐matis. Both IS 31831 and IS 1096 exhibit several common characteristics suggesting that they constitute a new family of insertion sequences. IS 31831 was isolated by taking advantage of the sucrose sensitivity of coryneform bacteria conferred by expression of the Bacillus subtilis sacB gene. An Escherichia coli/ Corynebacterium shuttle vector useful for the isolation of transposable elements from the coryneform group of bacteria was constructed.