Complete mitochondrial DNA sequence of the Lake Biwa wild strain of common carp (Cyprinus carpio L.):: further evidence for an ancient origin
Complete mitochondrial DNA sequence of the Lake Biwa wild strain of common carp (Cyprinus carpio L.):: further evidence for an ancient origin
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DOI：
10.1016/j.aquaculture.2006.03.040
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发表时间：
2006-06-30
期刊：
影响因子：
4.5
通讯作者：
Nishida, Mutsumi
中科院分区：
文献类型：
--
作者：
Mabuchi, Kohji;Miya, Masaki;Nishida, Mutsumi
采用PCR方法测定了日本琵琶湖野生鲤鱼（LBW）的线粒体DNA（mtDNA）全序列，并利用已发表的不同品系多基因区mtDNA序列重新研究了LBW品系在鲤鱼中的系统发育地位，从而对东南亚（越南和印度尼西亚）的其他品系进行了地理上更全面的分析。以下三个数据集，数据集A [部分D环区域序列（ca. B [来自39个菌株的ATP酶6和8基因序列（826 bp）]和C（来自9个菌株的COII基因序列（690 bp）），并使用金鱼（Carassius auratus auratus）作为外群进行系统发育分析。在所有的分析中，LBW和“欧亚”株，这是最近发现的基于cyt B基因和D-环区序列的主要系统发育二分法，被恢复。然而，“欧亚”（包括日本观赏锦鲤）菌株之间的系统发育关系，没有可靠的重建。低水平的核苷酸分歧，整个线粒体基因组，这是揭示了整个mtDNA之间的LBW和推定的台湾菌株的比较，表明没有一个单一的编码/非编码区可以提供足够的信息来解决亲缘关系密切的“欧亚”菌株的物种。(c)2006 Elsevier B. V.保留所有权利。
The complete mitochondrial DNA (mtDNA) sequence of Lake Biwa wild common carp (LBW) was determined using a PCR-based method, and the phylogenetic status of the LBW strain within common carp was re-examined using a number of already-published mtDNA sequences of multiple gene regions from various strains, which enabled a geographically more comprehensive analysis including additional strains from South-East Asia (Vietnam and Indonesia). The following three datasets, dataset A [partial D-loop region sequences (ca. 760 bp) from 44 strains], B [ATPase6 and 8 gene sequences (826 bp) from 39 strains] and C (COII gene sequences (690 bp) from 9 strains), were constructed from different studies, and phylogenetic analyses conducted using a goldfish (Carassius auratus auratus) as an outgroup. In all the analyses, a principal phylogenetic dichotomy between the LBW and "Eurasian" strains, which was recently revealed based on cyt b gene and D-loop region sequences, was recovered. Phylogenetic relationships among "Eurasian" (including Japanese ornamental koi) strains were, however, not reconstructed reliably. Low level nucleotide divergence throughout the mitochondrial genome, which was revealed by the whole mtDNA comparison between the LBW and a putative Taiwanese strain, indicated that no single coding/non-coding region could provide sufficient information for resolving phylogeny among the closely-related "Eurasian" strains of the species. (c) 2006 Elsevier B.V. All rights reserved.