Methionine synthase activities in mice following acute exposures to ethanol and nitrous oxide.
Methionine synthase activities in mice following acute exposures to ethanol and nitrous oxide.
复制标题
急性暴露于乙醇和一氧化二氮后小鼠的蛋氨酸合酶活性。
DOI：
10.1016/0006-2952(89)90343-2
复制
发表时间：
1989
影响因子：
5.8
通讯作者：
Tomerson,BW
中科院分区：
文献类型：
--
作者：
Koblin,DD;Tomerson,BW
急性或慢性暴露于一氧化二氮或慢性暴露于乙醇会降低维生素B 12依赖性蛋氨酸合酶的活性。为了评估急性暴露于一氧化二氮和乙醇的综合影响，小鼠给予腹腔注射乙醇（3克/公斤），并暴露于吸入的66%一氧化二氮和34%的氧气的混合物4 hr. Methylidine合成酶活性在肝脏，肾脏和大脑中的测定后立即暴露于一氧化二氮，并在不同的时间超过4天的恢复期。失活后2-4天，肝脏和肾脏中的甲硫氨酸合酶活性恢复到对照水平。在大脑中，甲硫氨酸合酶活性显着下降16%后，4天的恢复期。急性给药乙醇并没有改变一氧化二氮诱导的失活的幅度，也没有失活后蛋氨酸合酶活性恢复的时间过程。此外，在未暴露于一氧化二氮的小鼠中，单独或与0.4%大气压异氟烷联合急性给药均未改变蛋氨酸合酶活性。因此，在该动物模型中，急性剂量的乙醇不会改变甲硫氨酸合酶活性，也不会增强由一氧化二氮产生的失活。
Acute or chronic exposure to nitrous oxide or chronic exposure to ethanol decreases the activity of the vitamin B 12-dependent enzyme methionine synthase. To assess the combined effect of acute exposure to nitrous oxide and ethanol, mice were given an intraperitoneal injection of ethanol (3 g kg) and exposed to an inspired mixture of 66% nitrous oxide and 34% oxygen for 4 hr. Methionine synthase activities in liver, kidney, and brain were measured immediately after exposure to nitrous oxide and at various times over a 4-day recovery period. Methionine synthase activities in liver and kidney returned to control levels 2–4 days following inactivation. In brain, a significant 16% decrease in methionine synthase activity remained after a 4-day recovery period. The acute administration of ethanol did not alter the magnitude of the inactivation induced by nitrous oxide nor the time course of recovery of methionine synthase activity following inactivation. Moreover, in mice that were not exposed to nitrous oxide, methionine synthase activity was not altered by the acute administration of ethanol alone or in combination with 0.4% atm isoflurane. Thus, in this animal model, an acute dose of ethanol does not alter methionine synthase activity nor does it enhance the inactivation produced by nitrous oxide.