Microelectrode studies of the active Na transport pathway of frog skin
Microelectrode studies of the active Na transport pathway of frog skin
复制标题
青蛙皮肤活性钠转运途径的微电极研究
DOI：
--
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Fisher
中科院分区：
文献类型：
--
作者：
R. Fisher
当用微电极穿透短路青蛙皮肤的外表面时，持续记录到平均接近-100 mV的稳定负电位，证实了内格尔（W.内格尔。1975.第五届国际生物物理学大会摘要，哥本哈根。P-147.）。这些稳定电势V（O）的出现，同时观察到（a）占对照皮肤跨细胞阻力约75%或更多的高阻力外屏障R（O）已被穿透，（B）10（-5）M阿米洛利和减少的[Na]外导致V（O）值向平均值增加，接近-130 mV，而R（O）百分比值增加到90%以上。它的关系与在电流-电压关系的研究中观察到的E（1）的值相同，E '（1）的值被定义为当外部势垒的V（O）通过皮肤的电压钳位降低到零时内部势垒处的电压值。因此，这些数据被解释为意味着E（1）的值（约130 mV）代表钠泵在内屏障处的E（Na）。2，4-DNP使跨上皮电压降低小于E（1），V（O）为负值。这些数据可以用青蛙皮肤的主动钠转运途径的简单电等效电路来解释，该电路包括外膜作为离子转运的电整流器的想法。
When the outer surface of short-circuited frog skin was penetrated with microelectrodes, stable negative potentials that averaged near -100 mV were recorded consistently, confirming the results of Nagel (W. Nagel. 1975. Abstracts of the 5th International Biophysics Congress, Copenhagen. P-147.). The appearance of these stable potentials, V(O), concurrent with the observations that (a) a high resistance outer barrier R(O) accounting for approximately 75 percent or more of the transcellular resistance of control skins had been penetrated and that (b) 10(-5) M amiloride and reduced [Na] outside caused the values of V(O) to increase towards means value near -130 mV while the values of percent R(O) increased to more than 90 percent. It was of relationships were the same as the values of E(1) observed in studies of the current-voltage relationships were the same as the values of E’(1) defined as the values of voltage at the inner barrier when the V(O) of the outer barrier was reduced to zero by voltage clamping of the skins. Accordingly, these data are interpreted to mean that the values of E(1), approximately 130 mV, represent the E(Na) of the sodium pump at the inner barrier. 2,4-DNP was observed to decrease the values of transepithelial voltage less than E(1) the V(O) was negative. These data can be interpreted with a simple electrical equivalent circuit of the active sodium transport pathway of the frog skin that includes the idea that the outer membrane behaves as an electrical rectifier for ion transport.