Reconstruction of lactate utilization system in Pseudomonas putida KT2440: a novel biocatalyst for l-2-hydroxy-carboxylate production.
Reconstruction of lactate utilization system in Pseudomonas putida KT2440: a novel biocatalyst for l-2-hydroxy-carboxylate production.
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DOI：
10.1038/srep06939
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发表时间：
2014-11-06
影响因子：
4.6
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Wang Y;Lv M;Zhang Y;Xiao X;Jiang T;Zhang W;Hu C;Gao C;Ma C;Xu P
As an important method for building blocks synthesis, whole cell biocatalysis is hindered by some shortcomings such as unpredictability of reactions, utilization of opportunistic pathogen, and side reactions. Due to its biological and extensively studied genetic background, Pseudomonas putida KT2440 is viewed as a promising host for construction of efficient biocatalysts. After analysis and reconstruction of the lactate utilization system in the P. putida strain, a novel biocatalyst that only exhibited NAD-independent d-lactate dehydrogenase activity was prepared and used in l-2-hydroxy-carboxylates production. Since the side reaction catalyzed by the NAD-independent l-lactate dehydrogenase was eliminated in whole cells of recombinant P. putida KT2440, two important l-2-hydroxy-carboxylates (l-lactate and l-2-hydroxybutyrate) were produced in high yield and high optical purity by kinetic resolution of racemic 2-hydroxy carboxylic acids. The results highlight the promise in biocatalysis by the biotechnologically important organism P. putida KT2440 through genomic analysis and recombination.
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影响因子：
2.9
作者：
Das, Shuvendu;Glenn, James H.;Subramanian, Mani
通讯作者：
Subramanian, Mani
DOI：
10.1073/pnas.97.17.9413
发表时间：
2000-08-15
影响因子：
11.1
作者：
Dym, O;Pratt, EA;Eisenberg, D
通讯作者：
Eisenberg, D
影响因子：
3.2
作者：
del Castillo, Teresa;Ramos, Juan L.
通讯作者：
Ramos, Juan L.
影响因子：
3.2
作者：
Gao, Chao;Hu, Chunhui;Xu, Ping
通讯作者：
Xu, Ping
影响因子：
4.4
作者：
Gao, Chao;Zhang, Wen;Xu, Ping
通讯作者：
Xu, Ping