Side-entry excitation and detection of square capillary array electrophoresis for DNA sequencing.
Side-entry excitation and detection of square capillary array electrophoresis for DNA sequencing.
复制标题
用于 DNA 测序的方形毛细管阵列电泳的侧入激发和检测。
DOI：
10.1016/s0021-9673(99)00676-7
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
E. Yeung
中科院分区：
文献类型：
--
作者：
S. X. Lu;E. Yeung
在基于毛细管电泳的高通量 DNA 测序中，激光与每个毛细管的有效耦合是一个挑战。我们小组之前报道了两种多点照射方案。目前的工作描述了一种更有效的激发和检测方法，其中激光通过毛细管阵列传播，而不会严重降低功率。方形毛细管阵列（外径 340 μm × 内径 75 μm）夹在两个熔融石英板之间，中间有折射率匹配溶液。光从侧面穿过毛细管阵列进入通道。即使使用功率相对较低的激光，也能从 24 个毛细血管中获得 PGEM/U 的 DNA 序列。该激发方案可扩展到数百个毛细血管，以实现高速、高通量 DNA 测序、基因分型和药物筛选。
In high throughput DNA sequencing based on capillary electrophoresis, efficient coupling of the laser to each capillary is a challenge. Our group previously reported two multiple point irradiation schemes. The present work describes a more efficient excitation and detection method in which the laser light propagates through the capillary array without undergoing a serious reduction in power. An array of square capillaries (340 μm O.D.×75 μm I.D.) was sandwiched between two fused-silica plates with an index-matching solution in between. The light was directed into the channel across the capillary array from the side. DNA sequences of PGEM/U from 24 capillaries were obtained even with a relatively low-power laser. The excitation scheme can be scaled up to hundreds of capillaries to achieve high-speed, high-throughput DNA sequencing, genetic typing and drug screening.