Detection of polymorphisms in the 5'-flanking region of the gene for apolipoprotein(a).
Detection of polymorphisms in the 5'-flanking region of the gene for apolipoprotein(a).
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检测载脂蛋白 (a) 基因 5 侧翼区域的多态性。
DOI：
10.1006/bbrc.1995.1513
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Kuriyama,M
中科院分区：
文献类型：
--
作者：
Ichinose,A;Kuriyama,M
血浆中脂蛋白(a)的浓度以常染色体共显性方式遗传，其高浓度会导致动脉粥样硬化血栓性疾病。载脂蛋白(a)基因的核苷酸序列分析揭示了其5'侧翼区域存在多态性，这可以通过使用基因特异性引物的体外扩增进行分析。根据限制性消化的模式，正常个体的基因组 DNA 被细分为四种等位基因。白种人和日本人以及心肌梗塞患者中这些亚型的比例有所不同。 5'-等位基因很可能部分控制血浆脂蛋白(a) 水平。
Concentration of lipoprotein(a) in plasma is inherited in an autosomal co-dominant manner and its high concentration leads to atherothrombotic disease. Nucleotide sequence analysis of the apolipoprotein(a) gene revealed the presence of polymorphisms in its 5′-flanking region, which can be analyzed by in vitro amplification employing gene-specific primers. Genomic DNAs from normal individuals have been subclassified into four alleles according to the patterns of restriction digestion. The ratios of these subtypes differed between Caucasians and Japanese and in patients with myocardial infarction. It is very likely that the 5′-alleles in part control the plasma lipoprotein(a) level.