Spinal Cord Ventral Horns and Lymphoid Organ Involvement in Powassan Virus Infection in a Mouse Model.
Spinal Cord Ventral Horns and Lymphoid Organ Involvement in Powassan Virus Infection in a Mouse Model.
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DOI：
10.3390/v8080220
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发表时间：
2016-08-12
期刊：
影响因子：
--
通讯作者：
Thangamani S
中科院分区：
文献类型：
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作者：
Santos RI;Hermance ME;Gelman BB;Thangamani S
波瓦桑病毒 (POWV) 属于黄病毒科，是蜱传脑炎血清群的成员。美国、加拿大和俄罗斯已记录了 POWV 从受感染蜱传播给人类的情况，导致 10% 的人类病例出现致命性脑炎，并给幸存者带来严重的神经系统后遗症。我们使用 C57BL/6 小鼠来研究 POWV 感染和发病机制。足垫接种后，受感染的动物表现出快速的疾病进展和 100% 的死亡率。免疫组织化学和免疫荧光显示POWV感染具有非常强的神经元趋向性。中枢神经系统感染表现为脑膜脑炎，伴有血管周围单核细胞浸润和小胶质细胞活化，以及脊髓腹角POWV抗原水平高的脊髓灰质炎样综合征。病理学研究还揭示了POWV对脾脏巨噬细胞的大量感染，这表明脾脏在发病机制中发挥着比之前认识到的更重要的作用。本报告详细描述了 C57BL/6 小鼠 POWV 感染产生的病变的神经解剖学分布。
Powassan virus (POWV) belongs to the family Flaviviridae and is a member of the tick-borne encephalitis serogroup. Transmission of POWV from infected ticks to humans has been documented in the USA, Canada, and Russia, causing fatal encephalitis in 10% of human cases and significant neurological sequelae in survivors. We used C57BL/6 mice to investigate POWV infection and pathogenesis. After footpad inoculation, infected animals exhibited rapid disease progression and 100% mortality. Immunohistochemistry and immunofluorescence revealed a very strong neuronal tropism of POWV infection. The central nervous system infection appeared as a meningoencephalitis with perivascular mononuclear infiltration and microglial activation in the brain, and a poliomyelitis-like syndrome with high level of POWV antigen at the ventral horn of the spinal cord. Pathological studies also revealed substantial infection of splenic macrophages by POWV, which suggests that the spleen plays a more important role in pathogenesis than previously realized. This report provides a detailed description of the neuroanatomical distribution of the lesions produced by POWV infection in C57BL/6 mice.