Sulfhydryl/disulfide forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Sulfhydryl/disulfide forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.
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发表时间：
1985-12
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通讯作者：
G. Ness;M. J. Mccreery;C. Sample;M. Smith;L. Pendleton
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作者：
G. Ness;M. J. Mccreery;C. Sample;M. Smith;L. Pendleton
使用辐射灭活和免疫印迹技术，获得了分子量约为 100,000 和 200,000 的大鼠肝脏 3-羟基-3-甲基戊二酰辅酶 A 还原酶的功能活性形式的证据。在从同时喂食美维诺林和考来替泊的大鼠中分离出的肝微粒体中，Mr 100,000 形式是主要形式，而在来自仅饲喂考来替泊的动物的微粒体中，Mr 200,000 形式是主要形式。通过添加二硫苏糖醇或β-巯基乙醇，可以将该Mr 200,000 形式转化为Mr 100,000 形式。尽管两种形式似乎都具有催化活性，但 Mr 200,000 物质相对于 NADPH 浓度表现出 S 形动力学，而 Mr 100,000 形式则表现出典型的双曲线动力学。
Using radiation inactivation and immunoblotting techniques, evidence for functionally active forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase with molecular weights of about 100,000 and 200,000 was obtained. In liver microsomes isolated from rats fed both mevinolin and colestipol, the Mr 100,000 form was the predominant species, whereas in microsomes from animals fed only colestipol, the Mr 200,000 species was the major form. This Mr 200,000 form could be converted to the Mr 100,000 form by addition of dithiothreitol or beta-mercaptoethanol. Although both forms appear to possess catalytic activity, the Mr 200,000 species displays sigmoidal kinetics with respect to the concentration of NADPH, whereas the Mr 100,000 form exhibits typical hyperbolic kinetics.