Antioxidant activity of free and hydrolyzed phenolic compounds in soluble and insoluble dietary fibres derived from hulless barley
Antioxidant activity of free and hydrolyzed phenolic compounds in soluble and insoluble dietary fibres derived from hulless barley
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无壳大麦可溶性和不溶性膳食纤维中游离和水解酚类化合物的抗氧化活性
DOI：
10.1016/j.lwt.2019.05.086
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发表时间：
2019-08-01
影响因子：
6
通讯作者：
Zhang, Jianping
中科院分区：
文献类型：
--
作者：
Li, Qing;Yang, Shihua;Zhang, Jianping
与膳食纤维相关的酚类化合物以游离、可溶性共轭和不溶结合形式存在。本研究从无壳大麦的不溶性膳食纤维（IDF）和可溶性膳食纤维（SDF）中分别提取了总可溶性、游离、酯化、醚化和不溶性结合的酚类组分。对芦笋和芦笋5个酚类组分的总酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性进行了评价。主要酚酸和黄酮类化合物采用高效液相色谱法进行分析。结果表明，IDF 5个酚类组分的TPC和TFC分别为2.42 ~ 7.33倍和3.08 ~ 10.00倍，均高于SDF；不溶性结合酚组分的TPC和TFC在5个酚组分中最高。各酚类部位的TPC与抗氧化活性均呈显著的线性正相关（P < 0.05）。此外，阿魏酸是IDF和SDF中含量丰富的总可溶性和不溶性结合酚类化合物。本研究表明，IDF和SDF含有丰富的酚类化合物，可作为功能性食品成分或营养保健品。
Phenolic compounds associated with dietary fibre are present in free, soluble conjugated and insoluble-bound forms. In this study, total soluble, free, esterified, etherified and insoluble-bound phenolic fractions were extracted from the insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) of hulless barley. Total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of five phenolic fractions of IDF and SDF were evaluated. Major phenolic acids and flavonoids were analyzed by high performance liquid chromatography. The results revealed that the TPC and TFC of five phenolic fractions of IDF were 2.42-7.33 and 3.08-10.00 times, respectively, which were higher than those in SDF. Insoluble-bound phenolic fractions had the highest TPC and TFC among the five phenolic fractions. A significant positive linear correlation between TPC and antioxidant activity was observed in each of the five phenolic fractions from IDF and SDF (P < 0.05). Furthermore, ferulic acid was an abundant phenolic compound in total soluble and insoluble-bound phenolic fractions among IDF and SDF. This study indicates that IDF and SDF are rich in phenolic compounds which can be considered as functional food ingredients or nutraceuticals.