Developmental changes and the impact of isoflavones on mRNA expression of IGF-I receptor, EGF receptor and related growth factors in porcine skeletal muscle cell cultures
Developmental changes and the impact of isoflavones on mRNA expression of IGF-I receptor, EGF receptor and related growth factors in porcine skeletal muscle cell cultures
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DOI：
10.1016/j.ghir.2008.03.002
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发表时间：
2008-10-01
影响因子：
1.4
通讯作者：
Rehfeldt, Charlotte
中科院分区：
文献类型：
--
作者：
Kalbe, Claudia;Mau, Marcus;Rehfeldt, Charlotte
目的：大豆是大豆酮的丰富来源，广泛应用于人类营养和畜牧业生产。除了雌激素或抗雌激素作用外，还提示马诺酮通过抑制蛋白酪氨酸激酶（例如生长因子受体）影响细胞生长。因此，本研究在体外进行，以确定是否染料木素和大豆苷元影响生长因子受体的mRNA表达（IGF-I受体和EGF受体）及其相关生长因子在猪骨骼肌细胞培养物中的表达。首先，我们研究了IGF-I，IGF-II，EGF，IGF-I受体，和EGF受体在猪骨骼肌细胞增殖和分化中的作用。其次，我们测量了用0（对照）处理的这些细胞培养物中mRNA表达的变化。结果：与猪肌细胞增殖期相比，猪肌细胞分化期IGF-I的mRNA表达水平略有下降，而IGF-II和EGF的转录水平有所升高。IGF-I受体的转录本有增加的趋势，而EGF受体mRNA的表达从增殖到分化保持不变。染料木黄酮和大豆黄酮在1 μ M和10 μ M的这些基因的mRNA表达没有表现出影响，无论是在增殖，也不是在分化细胞。然而，高浓度的藜芦酮（100 μ M）降低IGF-I受体的mRNA表达和生长因子的examined.Conclusions：本研究证实了IGF和EGF系统在骨骼肌细胞培养的增殖和分化，特别是在无血清培养条件下的作用。此外。该体外研究的结果表明，在来自饮食消耗的浓度（1和10 μ M）下，对猪骨骼肌组织中IGF-和EGF-相关基因表达没有影响。染料木素和大豆苷元Lit高浓度（100 μ M）降低IGF-I受体和生长因子的mRNA表达的检查，因此，可能会修改其自分泌和旁分泌的行动在骨骼肌组织。(C)2008爱思唯尔有限公司保留所有权利。
Objective: Soy that is widely used in human nutrition and in livestock production is a rich source of isoflavones. In addition to the estrogenic or antiestrogenic effects, isoflavones are suggested to affect cell growth via inhibition of protein tyrosine kinases (e.g. growth factor receptors). Therefore, the present in vitro-study was undertaken to determine, whether genistein and daidzein affect the mRNA expression of growth factor receptors (IGF-I receptor and EGF receptor) and their related growth factors in porcine skeletal muscle cell cultures.Design: First, we investigated the basal mRNA expression of IGF-I, IGF-II, EGF, IGF-I receptor, and EGF receptor in proliferating and differentiating porcine skeletal muscle cell cultures using real-time PCR. Secondly, we measured the changes in the mRNA expression in these cell cultures treated with 0 (control). 1, 10, 100 mu M genistein or daidzein over 26 h in serum-free medium (n = 3).Results: The mRNA expression of IGF-I was slightly decreased, whereas transcript concentrations of IGF-II and EGF were increased during differentiation compared with the proliferating stage of porcine muscle cell cultures. IGF-I receptor transcripts tended to be increased, whereas EGF receptor mRNA expression remained unchanged from proliferation to differentiation. Genistein and daidzein at 1 mu M and 10 mu M showed no effects on the mRNA expression of these genes, neither in proliferating nor in differentiating cells. However, high-concentrated isoflavones (100 mu M) decreased the mRNA expression of IGF-I receptor and of the growth factors examined.Conclusions: The present study confirms the role of the IGF and EGF system in proliferation and differentiation of skeletal muscle cell culture especially under serum-free culture conditions. Furthermore. the results of this in vitro-study suggest that there is no effect of isoflavones at concentrations resulting from dietary consumption (1 and 10 mu M) on IGF- and EGF-associated gene expression in porcine skeletal muscle tissue. Genistein and daidzein Lit high concentration (100 mu M) reduced the mRNA expression of the IGF-I receptor and the growth factors examined, and therefore, may modify their autocrine and paracrine actions in skeletal muscle tissue. (C) 2008 Elsevier Ltd. All rights reserved.