Development and evaluation of a fluorogenic 5′-nuclease assay to identify Marburg virus
Development and evaluation of a fluorogenic 5′-nuclease assay to identify Marburg virus
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DOI：
10.1006/mcpr.2001.0369
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发表时间：
2001-10-01
影响因子：
3.3
通讯作者：
Henchal, EA
中科院分区：
文献类型：
--
作者：
Gibb, TR;Norwood, DA;Henchal, EA
快速识别马尔堡病毒感染的能力对于迅速建立适当的屏障护理预防措施和限制疾病的进一步传播至关重要。为了确认马尔堡病毒的暴发，并将其与其他可能出现类似临床症状的疾病区分开来，需要一种快速、灵敏和特异的实验室诊断试验。对新设计的引物/探针组(MBGGP3)的敏感性和特异性进行了评价。根据检测极限实验确定，MBGGP3的灵敏度相当于或比先前设计的引物组高10-100倍。此外，MBGGP3检测能够检测到所有被测试的马尔堡病毒株，但对其他出血热和基因相关病毒的检测结果为阴性。本研究结果表明，MBGGP3引物/探针组具有较高的敏感性和特异性。此外，该分析与新兴的快速核酸分析平台兼容，因此可能被证明是控制和管理未来疫情的有用诊断工具。(C)2001年学术出版社。
The ability to rapidly recognize Marburg virus infections is critical to quickly institute proper barrier nursing precautions and limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is necessary to confirm outbreaks of Marburg virus and to distinguish it from other diseases that can present with similar clinical symptoms.A one-tube reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the identification of Marburg virus was developed and evaluated using the ABI PRISM(TM) 7700 Sequence Detection System and TaqMan(R) chemistry. The sensitivity and specificity of the newly designed primer/probe set (MBGGP3) was evaluated. MBGGP3 was equivalent to or 10-100-fold more sensitive than previously designed primer sets as determined by limit of detection experiments. In addition, the MBGGP3 assay was able to detect all strains of Marburg virus tested, but gave negative results with other haemorrhagic fever and genetically related viruses. The results of this study indicate that the MBGGP3 primer/probe set is both sensitive and specific. In addition, this assay is compatible with emerging rapid nucleic acid analysis platforms and therefore may prove to be a useful diagnostic tool for the control and management of future outbreaks. (C) 2001 Academic Press.