Fibroblasts and Mesenchymal Stromal/Stem Cells Are Phenotypically Indistinguishable.
Fibroblasts and Mesenchymal Stromal/Stem Cells Are Phenotypically Indistinguishable.
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DOI：
10.1159/000445096
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发表时间：
2016
影响因子：
2.4
通讯作者：
Hematti P
中科院分区：
文献类型：
--
作者：
Denu RA;Nemcek S;Bloom DD;Goodrich AD;Kim J;Mosher DF;Hematti P
来源于许多不同组织的人间充质基质/干细胞（MSC）的特征在于成纤维细胞样形态、某些细胞表面标志物的表达以及它们分化成脂肪细胞、软骨细胞和成骨细胞的能力。许多研究表明，MSC与成纤维细胞有许多共同的特征，然而，没有一组明确的表型特征可以区分这两种类型的细胞。我们使用了来自三种不同组织来源的四种成熟的人成纤维细胞株和来自两种不同组织来源的几种人MSC株来比较这些细胞的表型和免疫学特性。成纤维细胞株显示出与MSC相似的形态，表达与MSC相同的细胞表面标志物，并且还可以分化成脂肪细胞、软骨细胞和成骨细胞。此外，与MSC相似，这些成纤维细胞能够抑制T细胞增殖并调节巨噬细胞的免疫表型。我们还发现，间充质干细胞存款细胞外基质的胶原蛋白I型和纤连蛋白，并表达FSP 1的模式类似于成纤维细胞。根据目前公认的对培养的人MSC和成纤维细胞的定义，我们找不到任何可以区分MSC和成纤维细胞的免疫表型特征。
Human mesenchymal stromal/stem cells (MSCs), derived from many different tissues, are characterized by a fibroblast-like morphology, expression of certain cell surface markers, and their ability to differentiate into adipocytes, chondrocytes, and osteoblasts. A number of studies have shown that MSCs share many characteristics with fibroblasts, however, there is no well-defined set of phenotypic characteristics that could distinguish between these two types of cells. We used four well-established human fibroblast strains from three different tissue sources and several human MSC strains from two different tissue sources to compare phenotypic and immunological characteristic of these cells. Fibroblast strains show a morphology similar to MSCs, express the same cell surface markers as MSCs, and could also differentiate into adipocytes, chondrocytes, and osteoblasts. Also, similar to MSCs, these fibroblasts were capable of suppressing T cell proliferation and modulating the immunophenotype of macrophages. We also show that MSCs deposit extracellular matrices of collagen type-I and fibronectin, and express FSP1 in patterns similar to fibroblasts. Based on currently accepted definitions for cultured human MSCs and fibroblasts, we could not find any immunophenotypic property that could make a characteristic distinction between MSCs and fibroblasts.