A NEW PRESS FOR THE DISRUPTION OF MICRO-ORGANISMS AND OTHER CELLS
A NEW PRESS FOR THE DISRUPTION OF MICRO-ORGANISMS AND OTHER CELLS
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DOI：
10.1002/jbmte.390020409
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
EDEBO, L
中科院分区：
文献类型：
--
作者：
EDEBO, L
一种新的压力机（X-press）已经开发出来，用于在冷冻状态下（主要在-25 °C）分解细胞。通过迫使装在圆筒中的冷冻细胞通过一个比圆筒直径小得多但比细胞大小大得多的孔，可以获得令人满意的崩解效果。通过迫使细胞反复通过孔而不是一次，可以获得更高程度的崩解。通过使接收器与装料室相同，可以重复操作，而无需断开压机。破碎的材料非常容易回收--它简单地以圆柱体的形式从压机中取出，圆柱体中含有冷冻的、破碎的材料，然后容易地均质化。这一原理已被发现对大量细胞的破碎是满意的，即细菌、酵母、霉菌，植物和动物细胞，并且就生物化学问题而言，已经发现它上级不处理冷冻状态细菌的现有方法。并对崩解作用的机理进行了讨论。
A new press (the X‐press) has been developed for the disintegration of cells in the frozen state (mostly at −25°C). By forcing the frozen cells contained in a cylinder through a hole much smaller than the diameter of the cylinder but much larger than the size of the cells, a satisfactory disintegration can be obtained.By forcing the cells through the hole repeatedly rather than once, a higher degree of disintegration can be achieved. By making the receiver identical to the charge chamber, repeated operations are possible without uncoupling the press.The disintegrated material is very easily recovered—it is simply taken out of the press in the form of a cylinder containing the frozen, disintegrated material which is then easily homogenized.This principle has been found satisfactory for the disintegration of a large number of cells,viz.bacteria, yeasts, moulds, plant and animal cells and, with regard to biochemical questions, it has been found to be superior to existing methods which do not treat the bacteria in the frozen state. The mechanism of the disintegrating action is discussed.