DIRECT MEASUREMENT OF MICROVESSEL HEMATOCRIT, RED-CELL FLUX, VELOCITY, AND TRANSIT-TIME
DIRECT MEASUREMENT OF MICROVESSEL HEMATOCRIT, RED-CELL FLUX, VELOCITY, AND TRANSIT-TIME
复制标题
DOI：
10.1152/ajpheart.1982.243.6.h1018
复制
发表时间：
1982-01-01
影响因子：
--
通讯作者：
DULING, BR
中科院分区：
文献类型：
--
作者：
SARELIUS, IH;DULING, BR
提出了一种在体研究红细胞流动动力学的方法。该方法允许直接测量微血管血液中的红细胞体积分数，而无需借助体外校准曲线。此外，该方法不需要大量的数学运算，并且可以应用于任何组织中的任何微血管网络。该方法还能够直接测量红细胞速度、流量和毛细血管通过时间。以示踪剂量但已知浓度的双标记红细胞用作总细胞群行为的指示剂。红细胞通过血管网的时间和红细胞速度直接通过跟踪标记细胞的行为来确定。红细胞压积和红细胞流量通过标准微循环方法使用标记细胞而不是总细胞群测量。然后将数据从标记细胞的测量分数转换为绝对值。因此，该方法绝对依赖于流变学正常的标记细胞，并且定义了满足该要求的条件。通过使用这种方法获得的微血管数据仓鼠颊囊和提睾肌。
A method is presented for the in vivo study of red cell flow dynamics. The method permits direct measurement of the red cell volume fraction in microvessel blood without resort to in vitro calibration curves. Furthermore, the method does not require extensive mathematical manipulation and can be applied to any microvascular network in any tissue. The method also enables direct measurement of red cell velocity, flux, and capillary transit time. Fluorescently labeled erythrocytes in tracer quantities, but known concentrations, are used as indicators of the behavior of the total cell population. Erythrocyte transit time across vascular networks and erythrocyte velocity are determined directly by following the behavior of the labeled cells. Hematocrit and red cell flux are measured by standard microcirculatory methods using labeled cells instead of the total cell population. Data are then converted to absolute values from the measured fraction of labeled cells. The method is thus absolutely dependent on the labeled cells being rheologically normal, and the conditions under which this requirement is satisfied are defined. Microvascular data obtained by the use of this method are presented for hamster cheek pouch and cremaster muscle.