Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts
Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts
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DOI：
10.1046/j.1467-2494.1998.171747.x
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发表时间：
1998-06-01
影响因子：
2.3
通讯作者：
Bernard, B. A.
中科院分区：
文献类型：
--
作者：
Boyera, N.;Galey, I.;Bernard, B. A.
维生素C （VitC）通过防止赖氨酸和脯氨酸羟化酶（胶原生物合成中的两种关键酶）的自失活，在维持正常的成熟胶原网络（抗坏血病特性）中起着关键作用。在本研究中，我们设计了两个体外模型来评估维生素c在细胞和组织水平上对胶原生物合成和交联的影响。结果表明，在非细胞毒性浓度范围内（10-3 M, 10-4 M, 10-5 M），维生素c诱导单层培养的正常人成纤维细胞（NHF）中I型胶原沉积呈剂量依赖性增加，并增强晶格模型中NHF的细胞外基质收缩。因此，外源性维生素c的供应有助于维持真皮中最佳的胶原密度，并在局部加强胶原网络。在我们的两个模型中也测试了维生素c -磷酸（维生素c -p）和维生素c -葡萄糖苷（维生素c -glu）这两种维生素c衍生物在水溶液中具有较高的化学稳定性，它们的生物学特性相似，但效力不同。这两种化合物可以被认为是皮肤的维生素原，因此可以在以维生素c为基础的抗衰老产品中有利地替代维生素c，因为它们允许开发稳定，易于使用的配方。
Vitamin C (VitC) plays a critical role in the maintenance of a normal mature collagen network in humans (antiscurvy properties) by preventing the auto-inactivation of lysyl and prolyl hydroxylase, two key enzymes in collagen biosynthesis. In this study two in vitro models were designed to evaluate the effects of VitC on collagen biosynthesis and cross-linking at cellular and tissue levels. It was shown that VitC induced a dose-dependent increase in collagen type I deposits by normal human fibroblasts (NHF) cultured in monolayer, and enhanced extracellular matrix contraction by NHF in a lattice model, in a non-cytotoxic range of concentrations (10-3 M, 10-4 M, 10-5 M). Exogenous VitC supply could thus contribute to the maintenance of optimal collagenic density in the dermis and locally strengthen the collagen network. Vitamin C-phosphate (VitC-P) and vitamin C-glucoside (VitC-Glu) (two VitC derivatives presenting higher chemical stability in aqueous solution) were also tested in our two models, and showed similar biological properties, but with different potencies. These two compounds can be considered as pro-vitamins for skin, and could thus advantageously substitute for VitC in VitC-based anti-ageing products, as they allow the development of stable, easy-to-use formulations.