EST‐SSR markers from the Pacific oyster, Crassostrea gigas
EST‐SSR markers from the Pacific oyster, Crassostrea gigas
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DOI：
10.1111/j.1471-8286.2007.01729.x
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
Hong Yu;Qi Li
中科院分区：
文献类型：
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作者：
Hong Yu;Qi Li
从太平洋牡蛎（Crassostrea gigas）的表达序列标签（expressed sequence tags，EST）中筛选出10个多态性微卫星重复序列标记。每个位点的等位基因数在3 ~ 18个之间，期望杂合度在0.071 ~ 0.738之间，观测杂合度在0.306 ~ 0.913之间。在另外两种牡蛎上进行了标记可传递性测试，在9个位点上检测到多态性产物。EST衍生的简单重复序列为群体遗传、亲子鉴定和定位研究提供了可靠的、信息丰富的和潜在的多态性标记。千兆。
Ten polymorphic microsatellite repeat markers were identified from Crassostrea gigas, expressed sequence tags (EST) deposited in public sequence database. Number of alleles per locus ranged from three to 18, expected and observed heterozygosities ranged from 0.071 to 0.738 and from 0.306 to 0.913, respectively. Marker transferability was tested on other two Crassostrea species and polymorphic products were detected at nine loci. EST-derived simple sequence repeats provide robust, informative and potentially transferable polymorphic markers suitable for population genetic, parentage, and mapping studies of C. gigas.