Microchip electrospray: Cone-jet stability analysis for water-acetonitrile and water-methanol mobile phases
Microchip electrospray: Cone-jet stability analysis for water-acetonitrile and water-methanol mobile phases
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DOI：
10.1016/j.chroma.2011.01.054
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发表时间：
2011-03-25
影响因子：
4.1
通讯作者：
Tallarek, Ulrich
中科院分区：
文献类型：
--
作者：
Jung, Stephanie;Effelsberg, Uwe;Tallarek, Ulrich
高效液相色谱（HPLC）梯度洗脱与质谱（MS）联用过程中移动的相组成的变化敏感地影响电喷雾操作模式。在这项工作中，我们确定了动态变化的体积电导率上的锥形喷射稳定性岛的乙腈水溶液和甲醇水溶液移动的相通常用于反相HPLC的影响。通过加入不同量的甲酸改变移动的相的体积电导率。商业微芯片-HPLC/ESI-MS配置进行了修改，使原位电喷雾诊断的微芯片发射极电流和喷雾成像的频率分析。这种方法便于检测不同的喷雾模式连同它们的起始电位。所建立的喷雾模式进行了描述和解释的电喷雾液体的物理化学性质的起始电位和稳定区域的差异。(C)2011 Elsevier B. V.保留所有权利。
Changes in mobile phase composition during high performance liquid chromatography (HPLC) gradient elution coupled to mass spectrometry (MS) sensitively affect electrospray operation modes. In this work, we identify the influences of dynamic changes in bulk conductivity on the cone-jet stability island for aqueous acetonitrile and aqueous methanol mobile phases commonly used in reversed-phase HPLC. Bulk conductivities of the mobile phases were varied by adding different amounts of formic acid. A commercial microchip-HPLC/ESI-MS configuration was modified to enable in situ electrospray diagnostics by frequency analysis of the microchip emitter current and spray imaging. This approach facilitated the detection of different spray modes together with their onset potentials. The established spray modes are described and the differences in onset potentials and stability regions explained by the physicochemical properties of the electrosprayed liquid. (C) 2011 Elsevier B.V. All rights reserved.