Sciatic nerve regeneration in KLF7-transfected acellular nerve allografts
Sciatic nerve regeneration in KLF7-transfected acellular nerve allografts
复制标题
KLF7 转染的无细胞神经同种异体移植物中的坐骨神经再生。
DOI：
10.1080/01616412.2015.1105584
复制
发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Guan, Li-Xin
中科院分区：
文献类型：
--
作者：
Wang, Ying;Li, Wen-Yuan;Guan, Li-Xin
目的：Kruppel样因子7（KLF 7）是一种促进中枢神经系统轴突再生的转录因子。在这里，我们评估是否KLF 7刺激周围nerveinjuries.Methods后再生：C57 BL/6小鼠接受无细胞神经同种异体移植物（ANA）注射腺相关病毒2（AAV 2）载体或AAV 2-KLF 7坐骨神经间隙修复。4周后，采用RT-PCR、Western blot和免疫组织化学方法检测再生神经中KLF 7的表达。结果：注射AAV 2-KLF 7后，再生神经中KLF 7的表达增加，SFI的波幅、评分、踝关节角度和FG标记的脊髓神经元均增加。我们在Hargreaves测试中观察到背根神经节（DRG）、神经丝、P-0（外周髓鞘）和S100中CTB标记的神经元升高，潜伏期和戒断潜伏期缩短。SFI与波幅和再生轴突数显著相关。酪氨酸激酶A（TrkA）和B（Trk B）受体也增加在DRG.Conclusions：我们的研究结果表明，KLF 7促进周围神经轴突再生，进一步支持KLF 7作为一个生长促进转录因子在受损的神经系统中的作用。
Objective: Kruppel-like Factor 7 (KLF7) is a transcription factor that promotes axon regeneration in the central nervous system. Here, we assessed whether KLF7 stimulates regeneration after peripheral nerve injury.Methods: C57BL/6 mice received an acellular nerve allograft (ANA) injected with either adeno-associated virus 2 (AAV2) vector or AAV2-KLF7 for sciatic nerve gap repair. After 4 weeks, KLF7 was detected by RT-PCR, western blot and immunohistochemistry in regenerated nerves. Axonal regeneration and functional recovery were examined by immunohistochemistry, Fluorogold (FG) and cholera toxin B (CTB) retrograde neural tracing, sciatic function index (SFI), angle of ankle, Hargreaves test and electrophysiological analysis.Results: With AAV2-KLF7 injection, KLF7 expression increased in regenerated nerves, and amplitude, score of SFI, angle of ankle and FG-labelled spinal cord neurons were increased. We observed elevated CTB-labelled neurons in dorsal root ganglia (DRG), neurofilaments, P-0 (peripheral myelin) and S100 and decreased latency period and withdrawal latencies in the Hargreaves test. The SFI was significantly correlated with amplitude and regenerated axon number. Tyrosine kinase A (TrkA) and B (TrkB) receptors were also increased in the DRG.Conclusions: Our findings suggest that KLF7 promoted peripheral nerve axonal regeneration, further supporting a role for KLF7 as a growth-promoting transcription factor in the injured nervous system.