Characterization of Flowering-related Genes and Flowering Response in Relation to Blue Light in Gypsophila paniculata
Characterization of Flowering-related Genes and Flowering Response in Relation to Blue Light in Gypsophila paniculata
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DOI：
10.2503/hortj.mi-126
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
T. Shibuya;Y. Murakawa;K. Nishidate;M. Nishiyama;Y. Kanayama
中科院分区：
文献类型：
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作者：
T. Shibuya;Y. Murakawa;K. Nishidate;M. Nishiyama;Y. Kanayama
需要表征对单色光的开花反应以及该反应背后的开花相关基因，以有效地将发光二极管用于照明文化。长日照切花对远红光的开花反应已得到充分研究，但很少有研究调查对蓝光的反应。满天星（一种重要的长日照切花“布里斯托仙女”）在蓝光长日照条件下开花和 G. paniculata 同源物的 FLOWERING LOCUS T 和 SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 基因 (GpFT, GpSOC1) 的表达先前并未得到促进。在本研究中，我们发现另一种G.paniculata“百万星”在蓝光长日照条件下促进开花，这表明G.paniculata对蓝光的开花反应存在差异。因此，我们分析了‘百万星’中GpFT和GpSOC1的表达情况。与‘Bristol Fairy’相比，‘Million Star’在蓝光长日照条件下开花时 GpFT 和 GpSOC1 的表达得到促进。接下来，我们分析了 FLAVIN-BINDING KELCH REPEAT F-BOX 1 和 GIGANTEA 基因的 G. paniculata 同源物 (GpFKF1、GpGI)，它们参与蓝光的开花反应。 GpFKF1和GpGI氨基酸序列非常保守；正如之前在拟南芥中观察到的那样，基因表达在短日照和长日照条件下表现出具有不同峰值的昼夜节律。 GpFKF1 与 GpGI 相互作用。两个品种之间的 GpFKF1 或 GpGI 氨基酸序列没有显着差异。我们的结果表明，开花对蓝光反应的变化与 GpFT 和 GpSOC1 有关，而不是与 GpFKF1 和 GpGI 有关。
The flowering response to monochromatic light and flowering-related genes underlying this response need to be characterized to efficiently use light-emitting diodes for lighting culture. The flowering response to far-red light has been well studied in long-day cut flowers, but there have been few studies investigating the response to blue light. Flowering and the expression of the G. paniculata homologs of the FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 genes (GpFT, GpSOC1) were not previously promoted in Gypsophila paniculata, an important long-day cut flower, ‘Bristol Fairy’ under long-day conditions with blue light. In the present study, we found that flowering was promoted in another G. paniculata ‘Million Star’, under long-day conditions with blue light, suggesting that there is variation in G. paniculata’s flowering response to blue light. Therefore, we analyzed the expression of GpFT and GpSOC1 in the ‘Million Star’. The expression of GpFT and GpSOC1 was promoted with flowering in ‘Million Star’ under long-day conditions with blue light in contrast to ‘Bristol Fairy’. We next analyzed the G. paniculata homologs (GpFKF1, GpGI) of FLAVIN-BINDING KELCH REPEAT F-BOX 1 and GIGANTEA genes, which participate in the flowering response to blue light. GpFKF1 and GpGI amino acid sequences were well conserved; gene expression showed a diurnal rhythm with different peaks under short-day and long-day conditions, as previously observed in Arabidopsis thaliana. GpFKF1 interacted with GpGI. There were no important differences in GpFKF1 or GpGI amino acid sequences between the two cultivars. Our results suggest that variation in the flowering response to blue light is associated with GpFT and GpSOC1, rather than GpFKF1 and GpGI.