Elevated B cells in sputum of asthmatics - Close correlation with eosinophils
Elevated B cells in sputum of asthmatics - Close correlation with eosinophils
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DOI：
10.1164/ajrccm.153.2.8564094
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发表时间：
1996-02-01
影响因子：
24.7
通讯作者：
Hargreave, FE
中科院分区：
文献类型：
--
作者：
Kidney, JC;Wong, AG;Hargreave, FE
痰检查是研究气道炎症的一种有用的无创方法。我们研究了 11 名稳定的哮喘患者和 10 名非哮喘吸烟者痰液中淋巴细胞亚群测量的重现性和有效性。用0.1%二硫苏糖醇分散痰液。用赖特氏染色进行分类细胞计数。等分试样用 T 细胞亚群上的 CD19（B 细胞）、CD3（T 细胞）、CD4（辅助细胞）、CD8（抑制细胞）和激活标记 CD25（IL2 受体）的抗体进行染色，并通过流式细胞术进行分析。与非哮喘受试者相比，哮喘患者的痰液中嗜酸性粒细胞较多（平均值 +/- SEM，32.5 +/- 8.5 对比 1.3 +/- 0.5%，p < 0.01），并且 B 细胞淋巴细胞比例较高（16.2 +/- 3.2 对比 4.0 +/- 1.0%，p < 0.01），并且这些与嗜酸性粒细胞密切相关 （r = 0.8，p < 0.01）。哮喘患者也有更多活化的 T 辅助细胞（39.3 +/- 4.6 对比 9.0 +/- 9.0%，p = 0.05），但由于巨噬细胞自发荧光，比较仅限于两名吸烟者。辅助 T 细胞 (R = 0.94)、抑制性 T 细胞 (R = 0.88) 和活化辅助 T 细胞 (R = 0.77) 测量的重复性良好；无法检查 T 细胞和 B 细胞测量的重复性，因为它们是彼此的倒数。哮喘痰与非哮喘吸烟对照受试者的痰具有不同的淋巴细胞谱。结果证明了气道中产生抗体的淋巴细胞的潜在重要性及其与哮喘中痰液嗜酸性粒细胞增多的关系。
Sputum examination is a useful noninvasive method to study airway inflammation. We investigated the reproducibility and validity of the measurements of lymphocyte subsets in the sputum of 11 stable patients with asthma and 10 nonasthmatic smokers. Sputum was dispersed with 0.1% dithiothreitol. A differential cell count was made with Wright's stain. Aliquots were stained with antibodies to CD19 (B cells), CD3 (T cells), CD4 (helper), CD8 (suppressor), and the activation marker CD25 (IL2 receptor) on T-cell subsets and were assayed by flow cytometry. Sputum from patients with asthma compared with nonasthmatic subjects had more eosinophils (mean +/- SEM, 32.5 +/- 8.5 versus 1.3 +/- 0.5%, p < 0.01) and a higher proportion of lymphocytes that were B cells (16.2 +/- 3.2 versus 4.0 +/- 1.0%, p < 0.01), and these correlated closely with the eosinophils (r = 0.8, p < 0.01). Patients with asthma also had more activated T-helper cells (39.3 +/- 4.6 versus 9.0 +/- 9.0%, p = 0.05), but the comparison was limited to two smokers because of macrophage autofluorescence. The repeatability of measurements of helper T-cells (R = 0.94), suppressor T cells (R = 0.88), and activated helper T cells (R = 0.77) was good; repeatability of measurements of T and B cells could not be examined because these were reciprocals of each other. Asthmatic sputum has different lymphocyte profiles than sputum from nonasthmatic smoking control subjects. The results demonstrate a potential importance of antibody-producing lymphocytes in the airway and their relation to sputum eosinophilia in asthma.