A 2-YEAR STUDY OF THE DISTRIBUTION OF THERMOPHILIC CAMPYLOBACTERS IN HUMAN, ENVIRONMENTAL AND FOOD SAMPLES FROM THE READING AREA WITH PARTICULAR REFERENCE TO TOXIN PRODUCTION AND HEAT-STABLE SEROTYPE
A 2-YEAR STUDY OF THE DISTRIBUTION OF THERMOPHILIC CAMPYLOBACTERS IN HUMAN, ENVIRONMENTAL AND FOOD SAMPLES FROM THE READING AREA WITH PARTICULAR REFERENCE TO TOXIN PRODUCTION AND HEAT-STABLE SEROTYPE
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DOI：
10.1111/j.1365-2672.1989.tb04568.x
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发表时间：
1989-06-01
期刊：
影响因子：
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通讯作者：
PARK, RWA
中科院分区：
文献类型：
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作者：
FRICKER, CR;PARK, RWA
对食品和环境样本中“嗜热”弯曲杆菌的发病率进行了为期两年的研究。在781份环境样品中，发现529份（67%）含有弯曲杆菌，在2116份食物样品中，835份（39%）分离出弯曲杆菌。污水几乎总是被弯曲杆菌污染（96.6%的样品），家禽（55.5%）和内脏（47.0%）的食物样品通常被污染。从这些来源分离的所有菌株和从人粪便中分离的921株菌株的热稳定血清型的测定表明，在大多数类型的样品中有广泛的血清型分布。Pen2型是人类粪便中最常见的类型（18.9%），也是内脏（21.3%），牛肉（40.0%），污水（17.7%）中最常见的类型，是家禽中第三常见的类型。对细胞毒性肠毒素和细胞张力性肠毒素的最佳生产的培养基和条件的比较表明，在微需氧条件下于42 ℃孵育24小时的布鲁氏菌肉汤适合于两种毒素。细胞毒活性的检测是最敏感的HeLa细胞。两种酶联免疫吸附法和中国人卵巢组织培养法检测细胞张力性肠毒素的灵敏度相当。并非所有从人类肠炎病例中分离的菌株都产生毒素，23.1%产生细胞毒性肠毒素，17.5%产生细胞毒素。血清型与产毒无相关性。 弯曲杆菌的广泛分布，与从人类肠炎病例中分离出来的弯曲杆菌无法区分，这使我们得出结论，不应该做出简单的陈述，认为某种特定类型的食物是人类疾病的主要原因。
The incidence of ''thermophilic'' campylobacters in foods and environmental samples has been studied over a two-year period. Of 781 environmental samples, 529 (67%) were found to contain campylobacters, and campylobacters were isolated from 835 (39%) of 2116 food samples. Sewage was almost always contaminated with campylobacters (96.6% of samples) and of the food samples both poultry (55.5%) and offal (47.0%) were commonly contaminated. Determination of the heat-stable serotypes of all strains isolated from these sources and of 921 strains isolated from human faeces showed that there was a wide distribution of serotypes in most types of sample. Serotype Pen 2 was the commonest type found in human faeces (18.9%) and it was also commonest in offal (21.3%), beef (40.0%), sewage (17.7%) and was the third commonest type in poultry. A comparison of culture media and conditions for optimal production of both cytotoxic and cytotonic enterotoxins showed that Brucella Broth incubated under microaerobic conditions for 24 h at 42.degree.C was suitable for both toxins. Detection of cytotoxic activity was most sensitive using HeLa cells. The sensitivities of two ELISA systems and a Chinese Hamster Ovary tissue culture assay for detection of cytotonic enterotoxin were comparable. Not all strains isolated from cases of enteritis in human beings produced toxin; 23.1% produced cytotonic enterotoxin and 17.5% produced cytotoxin. There was no correlation between serotype and toxin production. The wide distribution of campylobacters, indistinguishable from those isolated from cases of enteritis in human beings, leads us to conclude that simplistic statements suggesting that one particular type of food is primarily responsible for cases of human disease should not be made.