Histological correlates of clinically occult human papillomavirus infection of the uterine cervix.
Histological correlates of clinically occult human papillomavirus infection of the uterine cervix.
复制标题
宫颈临床隐匿性人乳头瘤病毒感染的组织学相关性。
DOI：
10.1097/00000478-198803000-00005
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Crum,CP
中科院分区：
文献类型：
--
作者：
Nuovo,GJ;Nuovo,MA;Cottral,S;Gordon,S;Silverstein,SJ;Crum,CP
当从宫颈拭子中检测到病毒DNA序列但未检测到病变且巴氏涂片阴性时，则假定宫颈存在人乳头瘤病毒（HPV）的隐匿性感染。为了确定什么样的组织学变化与隐性感染相关，从200个子宫切除标本的宫颈拭子中提取DNA。DNA通过Southern印迹杂交分析HPV序列的存在。阳性11例（5.5%）。对每个病例以及28例阴性病例的整个宫颈进行组织学分析。阳性病例中有1例为CIN 2病变。另外10例显示角化不全、乳头状瘤、棘皮病以及局灶性核多形性和核周晕（边缘挖空细胞性核晕），比例与阴性对照相同。对显示临界挖空细胞异型性的病例进行原位杂交分析，结果均为阴性。这些结果证实，临床和细胞学上的宫颈隐匿性HPV感染与诊断性组织学变化无关。这强调了在解释宫颈活检时需要谨慎，这些活检显示的变化提示HPV感染，但不是绝对的诊断。此外，病毒的精确上皮位置仍不清楚。
Occult infection of the uterine cervix by human papillomavirus (HPV) is assumed when viral DNA sequences are detected from cervical swabs but no lesion is detectable and the Papanicolaou smear is negative. In an attempt to identify what histological changes correlate with occult infections, DNA was extracted from 200 cervical swabs taken from hysterectomy specimens. The DNA was analyzed by Southern blot hybridization for the presence of HPV sequences. Eleven cases (5.5%) were positive. The entire cervix from each case as well as from 28 negative cases was processed for histological analysis. One of the positive cases contained a CIN 2 lesion. The other 10 showed parakeratosis, papillomatosis, acanthosis, as well as focal nuclear pleomorphism and perinuclear halos (borderline koilocytotic atypia) in proportions equal to the negative controls. In situ hybridization analysis of the cases that showed borderline koilocytotic atypia were negative. These findings confirm that clinically and cytologically occult HPV infection of the uterine cervix is not associated with diagnostic histological changes. This underscores the need for caution when interpreting cervical biopsies that show changes suggestive, but not absolutely diagnostic, of HPV infection. Further, the precise epithelial location of the virus remains unclear.