Organization, structure and expression of murine interferon alpha genes.
Organization, structure and expression of murine interferon alpha genes.
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鼠干扰素α基因的组织、结构和表达。
DOI：
10.1093/nar/13.3.791
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发表时间：
1985
影响因子：
14.9
通讯作者：
J. Trapman
中科院分区：
文献类型：
--
作者：
E. Zwarthoff;A. Mooren;J. Trapman
使用人干扰素-α探针，我们已经从基因组文库中分离出含有鼠干扰素-α（Mu IFN-α）基因的重组质粒。其中一个包含两个完整的Mu IFN-α基因和第三个基因的一部分。第二个噬菌体的插入物含有两个IFN基因。这表明Mu IFN-α基因在基因组中成簇，类似于人类基因的情况。测定了这5个基因的核苷酸序列。他们表明，这些基因虽然高度同源，但都是不同的。推导的氨基酸序列表明，5个基因中的4个含有一个推定的糖基化位点。三个基因在COS细胞中瞬时表达，并产生具有抗病毒特性的蛋白产物。在病毒诱导的小鼠L细胞中研究了五种Mu IFN-α基因和Mu IFN-β基因的表达。在核酸酶S1实验中，使用每个基因的特异性探针观察单个mRNA。在来自诱导细胞的RNA制备物中，存在五种α基因和β基因中的每一种的mRNA。然而，观察到个体mRNA的量的实质性差异。
Using a human interferon-alpha probe we have isolated recombinant phages containing murine interferon-alpha (Mu IFN-alpha) genes from a genomic library. One of these phages contained two complete Mu IFN-alpha genes and part of a third gene. The insert of a second phage held two IFN genes. This indicates that the Mu IFN-alpha genes are clustered in the genome as is the case for the analogous human genes. The nucleotide sequences of these 5 genes were determined. They show that the genes are all different, albeit highly homologous. The deduced amino acid sequences show that four of the five genes contain a putative glycosylation site. Three genes were transiently expressed in COS cells and they gave rise to protein products showing antiviral properties. The expression of the five Mu IFN-alpha genes and the Mu IFN-beta gene was studied in virus-induced mouse L cells. The individual mRNAs were visualized in a nuclease S1 experiment, using a specific probe for each gene. In RNA preparations from induced cells mRNAs for each of the five alpha genes and the beta gene were present. However, substantial differences in the amounts of the individual mRNAs were observed.