Expression of extracellular matrix metalloprotease inducer in laryngeal squamous cell carcinoma
Expression of extracellular matrix metalloprotease inducer in laryngeal squamous cell carcinoma
复制标题
DOI：
10.1097/00005537-200308000-00027
复制
发表时间：
2003-08-01
期刊：
影响因子：
2.6
通讯作者：
Gladson, CL
中科院分区：
文献类型：
--
作者：
Rosenthal, EL;Shreenivas, S;Gladson, CL
目标/假设：头颈癌肿瘤细胞侵袭导致局部破坏和远处转移。侵袭主要由基质金属蛋白酶介导，基质金属蛋白酶被认为是由周围成纤维细胞中肿瘤细胞衍生的细胞外基质金属蛋白酶诱导物（EMMPRIN）诱导的。我们假设 EMMPRIN 在喉癌中过度表达。研究设计：声门上型喉癌患者的回顾性分析。方法：对正常和恶性组织进行总蛋白免疫印迹和免疫组织化学分析以确定EMMPRIN的表达。 33 名患者的 EMMPRIN 免疫反应性与临床病理特征和生存率相关。结果：通过免疫印迹检测，肿瘤 (n=8) 和转移淋巴结 (n=2) 的全组织裂解物表达显着量的 EMMPRIN，但正常皮肤 (n=8) 或粘膜 (n=6) 不表达。 EMMPRIN 膜免疫反应性（跨膜 EMMPRIN 评分）与淋巴结阳性相关（P=0.07），并且是生存的临界显着预测因子（风险比=2.4；95% CI，0.88-6.55）。作为分类变量，较高的跨膜 EMMPRIN 评分与较高的死亡率相关。结论：本研究有助于确定 EMMPRIN 是一种在发育不良粘膜和声门上喉癌中广泛表达的蛋白，但在正常上皮对应物中不表达。
Objectives/Hypothesis: Head and neck cancer tumor cell invasion is responsible for both local destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesize that EMMPRIN is overexpressed in laryngeal cancer. Study Design: Retrospective analysis of patients with supraglottic laryngeal cancer. Methods: Total protein immunoblotting and immunohistochemical analysis of normal and malignant tissue were performed to determine EMMPRIN expression. EMMPRIN immunoreactivity in 33 patients was correlated with clinicopathological features and survival. Results: Whole-tissue lysates of tumors (n=8) and metastatic lymph nodes (n=2), but not normal skin (n=8) or mucosa (n=6), expressed significant amounts of EMMPRIN by immunoblotting. EMMPRIN membrane immunoreactivity (transmembrane EMMPRIN score) was associated with nodal positivity (P=.07), and it was a borderline significant predictor of survival (Hazards Ratio=2.4; 95% CI, 0.88-6.55). As a categorical variable, higher transmembrane EMMPRIN score was associated with higher mortality. Conclusions: The present study helps to establish EMMPRIN as a widely expressed protein in dysplastic mucosa and supraglottic laryngeal cancer, but not in normal epithelial counterparts.