IN VITRO TRANSFORMATION OF PNEUMOCOCCAL TYPES : I. A TECHNIQUE FOR INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES IN VITRO.
IN VITRO TRANSFORMATION OF PNEUMOCOCCAL TYPES : I. A TECHNIQUE FOR INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES IN VITRO.
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DOI：
10.1084/jem.54.5.681
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发表时间：
1931-10-31
期刊：
影响因子：
--
通讯作者：
Sia RH
中科院分区：
文献类型：
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作者：
Dawson MH;Sia RH
1. 型特异性S型肺炎球菌可以通过体外方法完全从一种特定S型转化为其他特定S型。2. 由一种特定S型衍生而来的R型肺炎球菌可通过以下体外程序转化为其他特定S型：在含有由异源S型培养物制备的疫苗的培养基中培养R型的小接种。3. 在这个过程中，可以通过使用少量的S疫苗来实现类型转化，少量的S疫苗代表原培养物中0.1 cc的细菌。4. 在培养基中加入抗r血清，最容易产生由这种方法诱导的型转化。然而，在不含抗r抗体的培养基中，型转化可能会发生。5. 先前关于S型转化疫苗性质的热特性的发现已得到证实和扩展。
1. Type-specific S pneumococci may be transformed from one specific S type into other specific S types entirely by in vitro methods. 2. R forms of pneumococci, derived from S forms of one specific type, may be transformed into S forms of other specific types by the following in vitro procedure:—the growth of small inocula of R forms in media containing vaccines prepared from heterologous S cultures. 3. Transformation of type may be effected in this procedure by the use of small quantities of S vaccine,—quantities representing the bacteria from as little as 0.1 cc. of the original culture. 4. Transformation of type, as induced by this procedure, is most readily effected by employing anti-R serum in the culture medium. Transformation of type may be effected, however, in media which do not contain anti-R antibodies. 5. Previous findings on the thermal characteristics of the property of S vaccines responsible for transformation of type have been confirmed and extended.