The INO80 complex is required for damage-induced recombination
The INO80 complex is required for damage-induced recombination
复制标题
DOI：
10.1016/j.bbrc.2007.02.036
复制
发表时间：
2007-04-13
影响因子：
3.1
通讯作者：
Seki, Masayuki
中科院分区：
文献类型：
--
作者：
Kawashima, Satoshi;Ogiwara, Hideaki;Seki, Masayuki
出芽酵母INO80复合体参与染色质结构重塑，SWR1复合体将H2A/H2B二聚体替换为变体二聚体H2A。Z (Htz1) / H2B。据报道，这些染色质重塑复合物共同含有Arp4（肌动蛋白相关蛋白）和肌动蛋白，并被招募到HO内切酶诱导的DNA双链断裂（DSB）位点。据报道，Ino80可以驱逐ho诱导的DSB周围的核小体；然而，它在ho诱导的DSB修复中没有明显的作用。本研究表明，INO80染色质重塑活性的一个重要因子Arp8参与了损伤诱导的姐妹染色单体重组和异等位基因之间的染色体间重组。相反，arp6突变体擅长重组，这表明SWRI复合物不促进重组。我们的数据表明，INO80复合体对染色质的重塑促进了DNA损伤时有效的同源重组。(c) 2007爱思唯尔公司版权所有。
The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroallales. In contrast, arp6 mutants are proficient for recombination, indicating that the SWRI complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages. (c) 2007 Elsevier Inc. All rights reserved.