Lectins homologous to those of monocotyledonous plants in the skin mucus and intestine of pufferfish, Fugu rubripes
Lectins homologous to those of monocotyledonous plants in the skin mucus and intestine of pufferfish, Fugu rubripes
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DOI：
10.1074/jbc.m301038200
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发表时间：
2003-06-06
影响因子：
4.8
通讯作者：
Suzuki, Y
中科院分区：
文献类型：
--
作者：
Tsutsui, S;Tasumi, S;Suzuki, Y
我们从河豚鱼（红鳍东方鲀）的皮肤粘液中鉴定出了河豚凝集素，这是一种新型甘露糖特异性凝集素。通过凝胶过滤和基质辅助激光解吸电离飞行时间质谱法和 SDS-PAGE 分析的分子量估计表明 pufflectin 是由 13 kDa 非共价相关亚基组成的同二聚体。全长 pufflectin cDNA 由 527 bp 组成，由开放阅读框推断出 116 个氨基酸残基。 Pufflectin 的氨基酸序列与任何已知的动物凝集素没有同源性。令人惊讶的是，pufflectin 与单子叶植物的甘露糖结合凝集素具有序列同源性，并且保留了这些植物凝集素的三个碳水化合物识别结构域中的两个。 pufflectin 基因在鳃、口腔壁、食道和皮肤中表达。此外，同工型仅存在于肠道中。 Pufflectin 与从河豚血浆中纯化的甘露糖结合凝集素不同。虽然 pufflectin 没有凝集所测试的五种细菌，但它被证明可以与寄生吸虫 Heterobothrium okamotoi 结合。这一发现表明 pufflectin 有助于河豚的寄生虫防御系统。
We have characterized pufflectin, a novel mannose-specific lectin, from the skin mucus of the pufferfish, Fugu rubripes. Molecular mass estimations by gel filtration and matrix-assisted laser desorption ionization time-of-flight mass spectrometry and the SDS-PAGE pattern suggest that pufflectin is a homodimer composed of non-covalently associated subunits of 13 kDa. The full-length pufflectin cDNA consists of 527 bp, with 116 amino acid residues deduced from the open reading frame. The amino acid sequence of pufflectin shows no homology with any known animal lectin. Surprisingly, pufflectin shares sequence homology with mannose-binding lectins of monocotyledonous plants and has conserved two of three carbohydrate recognition domains of these plant lectins. The pufflectin gene is expressed in gills, oral cavity wall, esophagus, and skin. In addition, an isoform occurs exclusively in the intestine. Pufflectin differs from mannose-binding lectins purified from the blood plasma of Fugu. Whereas pufflectin did not agglutinate five bacterial species tested, it was demonstrated to bind to the parasitic trematode, Heterobothrium okamotoi. This finding suggests that pufflectin contributes to the parasite-defense system in Fugu.