Estrogen and estrogen mimics contamination in water and the role of sewage treatment
Estrogen and estrogen mimics contamination in water and the role of sewage treatment
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DOI：
10.2166/wst.2000.0265
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发表时间：
2000-01-01
影响因子：
2.7
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
--
作者：
Matsui, S;Takigami, H;Shimizu, Y
将酵母雌激素筛选应用于污水处理工艺沃茨，以鉴定雌激素活性的存在，并调查处理过程中雌激素物质的归宿和行为。使用C18柱提取并浓缩水相中的疏水部分，以有效提取17 β-雌二醇（E2）和其他雌激素模拟物。在所有供试样品中观察到酵母筛选中β-半乳糖苷酶合成的明显剂量依赖性升高，表明这些样品具有雌激素样作用。然而，雌激素活性在处理过程中，特别是在生物过程中趋于降低。酵母雌激素筛选的E2当量的定量结果与通过ELISA测量的实际E2浓度进行比较。E2在原污水中占总雌激素的34%，而在最终污水中几乎占100%。通过对污水处理过程中沃茨的分析，发现人体雌激素是污水及其处理产物中雌激素活性的主要致因。虽然环境雌激素与青鸭对人类健康和生态系统的影响可能存在相关性的研究结果仍然是科学辩论和调查的焦点，但应在接收各种环境污染物的污水处理过程中建立适当的管理。
The yeast estrogen screen was applied to sewage treatment process waters to identify the presence of estrogenic activity and to investigate the fate and behavior of estrogenic substances through treatment. Hydrophobic fractions in the water phase were extracted and concentrated using C18 cartridges for the effective extraction of 17 beta -estradiol (E2) and other estrogen mimics. Clear dose-dependent elevation in the synthesis of P-galactosidase in the yeast screen was observed with all the samples tested, demonstrating that these samples were estrogenic. However, estrogenic activity tended to reduce during the treatment, especially in the biological process. Quantification results of the yeast estrogen screen in terms of E2 equivalent were compared with actual E2 concentrations measured by an ELISA. E2 occupied 34% of the whole estrogenicity in the raw sewage, while almost 100% in the final affluent. The analyses of the sewage treatment process waters revealed that human estrogens are major causative substances in terms of estrogenic activity in sewage and its treated affluent. Although findings of possible correlation of environmental estrogens with the Teal impact on human health and the ecosystem are still the focus of scientific debate and investigation, proper management should be established in the sewage treatment process which receives various environmental contaminants.