Suppressors of the ras2 mutation of Saccharomyces cerevisiae.
Suppressors of the ras2 mutation of Saccharomyces cerevisiae.
复制标题
酿酒酵母 ras2 突变的抑制剂。
DOI：
10.1093/genetics/113.2.247
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Tatchell,K
中科院分区：
文献类型：
--
作者：
Cannon,JF;Gibbs,JB;Tatchell,K
酿酒酵母菌含有两种酵母菌家族成员。具有theras2基因破坏的菌株不能在不可发酵的碳源上有效生长。这种生长缺陷可以通过基因外突变来抑制。我们已经分离出79个独立的抑制突变，其中68个被分配到5个位点中的一个。另外11个显性突变没有被分配到一个特定的位点。一些sra1和sra4以及所有sra3突变是不依赖于ras的，这使得缺乏功能性ras基因的酵母细胞能够生长。突变insra1、SRA3、sra4和sra6分别与his6、ino1、met3和ade6相关。一些突变体具有多效性表型，影响糖原积累、产孢、活力、呼吸能力和抑制两个细胞分裂周期突变cdc25和cdc35。提出的许多抑制基因的功能与hras激活腺苷酸环化酶的模型一致。
Saccharomyces cerevisiaecontains two members of therasgene family. Strains with disruptions of theRAS2gene fail to grow efficiently on nonfermentable carbon sources. This growth defect can be suppressed by extragenic mutations calledsra. We have isolated 79 independent suppressor mutations, 68 of which have been assigned to one of five loci. Eleven additional dominant mutations have not been assigned to a specific locus. Somesra1andSRA4and allSRA3mutations wereRASindependent, allowing growth of yeast cells that lack a functionalRASgene. Mutations insra1, SRA3, SRA4andsra6are linked tohis6, ino1, met3andade6, respectively. Somesramutants have pleitropic phenotypes that affect glycogen accumulation, sporulation, viability, respiratory capacity and suppression of two cell-division-cycle mutations,cdc25andcdc35. The proposed functions of many of the suppressor genes are consistent with the model in whichRASactivates adenylate cyclase.