Elliptical Polymer Brush Ring Array Mediated Protein Patterning and Cell Adhesion on Patterned Protein Surfaces
Elliptical Polymer Brush Ring Array Mediated Protein Patterning and Cell Adhesion on Patterned Protein Surfaces
复制标题
椭圆聚合物刷环阵列介导的蛋白质图案化和图案化蛋白质表面上的细胞粘附
DOI：
10.1021/am403808s
复制
发表时间：
2013-12-11
影响因子：
9.5
通讯作者：
Yang, Bai
中科院分区：
文献类型：
--
作者：
Liu, Wendong;Li, Yunfeng;Yang, Bai
提出了一种制备蛋白质椭圆环阵列的新方法。蛋白质阵列是通过将蛋白质共价接枝到聚合物刷环阵列上制备的，聚合物刷环阵列是通过胶体光刻去湿和表面引发原子转移自由基聚合（SI-ATRP）相结合的技术制备的。通过这种方法，蛋白质模式的参数，如高度，壁厚，周期，和两个椭圆环之间的距离，可以微调。此外，包含椭圆形蛋白质环阵列的样品可以在高达1 cm（2）的大面积上制备，并且环上的蛋白质保持其生物活性。纤维连接蛋白的环状和椭圆环状阵列（ERA）能促进细胞粘附，并可能对肌动蛋白细胞骨架的形成有积极作用。
This paper presents a novel method to fabricate elliptical ring arrays of proteins. The protein arrays are prepared by covalently grafting proteins to the polymer brush ring arrays which are prepared by the techniques combining colloidal lithography dewetting and surface initiated atom-transfer radical polymerization (SI-ATRP). Through this method, the parameters of protein patterns, such as height, wall thickness, periods, and distances between two elliptical rings, can be finely regulated. In addition, the sample which contains the elliptical protein ring arrays can be prepared over a large area up to 1 cm(2), and the protein on the ring maintains its biological activity. The as-prepared ring and elliptical ring arrays (ERAs) of fibronectin can promote cell adhesion and may have an active effect on the formation of the actin cytoskeleton.