Spatial clusters of constitutively active neurons in mouse visual cortex.
Spatial clusters of constitutively active neurons in mouse visual cortex.
复制标题
小鼠视觉皮层中组成性活跃神经元的空间簇。
DOI：
10.1007/s12565-015-0284-z
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Makino;K.;Funayama;K.;Ikegaya;Y.
新皮层神经元的网络由自发活动协调，自发活动的水平在神经元之间表现出高度的异质性，范围从低活动水平到非常高的活动水平，即使在同一网络中。高度活跃的神经元在大脑皮层中占很小的比例，并且混合在绝大多数具有低放电率的神经元的网络中。然而，人们对大脑皮层内这些高度活跃的细胞的空间排列知之甚少。在这里，我们可视化他们的空间分布标记c-Fos，神经元活动标记物，在小鼠初级视觉皮层。通过引入不需要任意阈值c-Fos阳性的能量和熵参数，我们发现，强烈的c-Fos表达神经元聚集在附近。团簇直径约为100 μm，2/3层的团簇直径小于5和6层。第1层神经元没有表现出c-Fos表达神经元的聚集模式。我们的新统计方法不受人类偏见的影响，因此广泛适用于评估任何粒子的空间偏差。
The networks of neocortical neurons are coordinated by spontaneous activity, the level of which exhibits high heterogeneity among neurons, ranging from low activity levels to very high activity levels, even in the same network. Highly active neurons represent a small proportion in the cerebral cortex and are mingled in a web of the vast majority of neurons with low firing rates. However, little is known about the spatial arrangement of these highly active cells within the cerebral cortex. Here, we visualized their spatial distribution by labeling them with c-Fos, a neuronal activity marker, in the mouse primary visual cortex. By introducing energy-like and entropy-like parameters that did not require arbitrary thresholds for c-Fos positivity, we found that strongly c-Fos-expressing neurons were clustered in the vicinity. The cluster size measured approximately 100 μm in diameter and was smaller in layer 2/3 than in layers 5 and 6. Layer 1 neurons did not exhibit a clustered pattern of c-Fos-expressing neurons. Our novel statistical approaches are not subject to human bias and are thus widely applicable to evaluate the spatial bias of any particles.