The double-stranded RNA genome segments of cytoplasmic polyhedrosis virus are independently transcribed
The double-stranded RNA genome segments of cytoplasmic polyhedrosis virus are independently transcribed
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DOI：
10.1128/jvi.41.1.326-329.1982
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发表时间：
1982-01
影响因子：
5.4
通讯作者：
R. Smith;Y. Furuichi
中科院分区：
文献类型：
--
作者：
R. Smith;Y. Furuichi
对昆虫细胞质多角体病毒基因组片段的体外转录进行了研究，以寻找协同控制的可能机制。在没有CTP的情况下，通过预培养启动了第一个转录周期，所有10个病毒基因组片段的启动是同步的。完成每个基因组转录本所需的时间与链的长度成正比，所有10个信使核糖核酸的合成摩尔量与其分子量成反比。所有核糖核酸的链延长速率约为6个核苷酸/S，综合结果表明，每个病毒基因组片段都是独立转录的，提示RNA链的延长，而不是启动，是细胞质多角体病毒体外合成的速度限制。
The in vitro transcription of the genome segments of insect cytoplasmic polyhedrosis virus was examined for a possible mechanism of coordinate control. Initiation of the first transcription cycle, primed by preincubation in the absence of CTP, was synchronous for all 10 viral genome segments. The time required for the completion of each genome transcript was proportional to the chain length, and all 10 mRNA's were synthesized in molar amounts inversely proportional to their molecular weights. The rate of chain elongation for all mRNA's was approximately 6 nucleotides per s. The combined results indicate that each of the viral genome segments is transcribed independently and suggest that RNA chain elongation, not initiation, is rate limiting for cytoplasmic polyhedrosis virus mRNA synthesis in vitro.