The Antiapoptotic Effect of Granulocyte Colony-stimulating Factor Reduces Infarct Size and Prevents Heart Failure Development in Rats
The Antiapoptotic Effect of Granulocyte Colony-stimulating Factor Reduces Infarct Size and Prevents Heart Failure Development in Rats
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DOI：
10.1159/000331711
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发表时间：
2011-01-01
影响因子：
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通讯作者：
Mill, Jose G.
中科院分区：
文献类型：
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作者：
Baldo, Marcelo P.;Davel, Ana P. C.;Mill, Jose G.
背景/目的。粒细胞集落刺激因子（G-CSF）减轻心肌梗死（MI）后心肌损伤，改善心功能。我们研究了梗死大鼠G-CSF的早期改变和慢性影响。方法。雄性Wistar大鼠（200-250g）于心肌梗死手术前7、3、1天分别给予药体（MI）或g - csf (MI- gcsf) （50 μ g/kg, sc）。术后1天和15天制作心肌梗死并测量梗死面积。在手术前立即评估抗和促凋亡蛋白的表达。术后24小时观察凋亡核的变化。心肌梗死后2周，评估左室（LV）功能，随后进行左室舒张压-容积原位评估。结果。闭塞前预处理1天梗死面积减小(心肌梗死36 +/- 2.8 vs.心肌梗死44 +/- 2.1%
Background/Aim. Granulocyte colony-stimulating factor (G-CSF) reduces myocardial injury and improves cardiac function after myocardial infarction (MI). We investigated the early alterations provided by G-CSF and the chronic repercussions in infarcted rats. Methods. Male Wistar rats (200-250g) received vehicle (MI) or G-CSF (MI-GCSF) (50 mu g/kg, sc) at 7, 3 and 1 days before MI surgery. Afterwards MI was produced and infarct size was measured 1 and 15 days after surgery. Expression of anti-and proapoptotic proteins was evaluated immediately before surgery. 24 hours after surgery, apoptotic nuclei were evaluated. Two weeks after MI, left ventricular (LV) function was evaluated, followed by in situ LV diastolic pressure-volume evaluation. Results. Infarct size was decreased by 1 day pretreatment before occlusion (36 +/- 2.8 vs. 44 +/- 2.1% in MI; P