Histochemical Changes of the Developing Human Cerebral Neocortex
Histochemical Changes of the Developing Human Cerebral Neocortex
复制标题
发育中的人类大脑新皮质的组织化学变化
作者：
E. Farkas‐Bargeton;M. Diebler;B. Rosenberg;R. Wehrlé
两种线粒体酶，琥珀酸和甲萘醌依赖性α-甘油磷酸脱氢酶（SDH，M α GPDH）的发育变化已通过组织化学技术在人类新皮质以下区域的早期尸检材料中进行了研究：区域4（运动皮质）、区域17（视觉皮质）和区域40（联想皮质）。研究的每个领域都揭示了一种特殊的酶结构。 SDH——氧化活性的标志——在出生后几年内发育，在某些地区（4岁和40岁）仅在11年后才达到成年模式。在发育过程中，不同皮质层的相对活动会发生相当大的变化。 SDH 活动最初出现在将投射发送到皮质下中继或接收特定丘脑皮质传入的层中。接受皮质-皮质投射的各层稍后成熟。与 SDH 活性相反，M α GPDH 在出生前就已经升高，直到 1 个月大时才增加，然后下降。这种存在于神经母细胞中的酶从成熟的皮质神经元中消失，第六层除外。这种酶在儿童皮质层中的分布与SDH相反。这两种酶的发育进化已通过生化测定得到证实。
Developmental changes of two mitochondrial enzymes, succinate- and menadione-dependent alpha-glycerophosphate dehydrogenases (SDH, M alpha GPDH) have been studied by histochemical techniques in early autopsy material in the following areas of human neocortex: area 4 (motor cortex), area 17 (visual cortex) and area 40 (associative cortex). Each area studied revealed a special enzymatic architecture. SDH - a marker of oxidative activity - develops for several years after birth and in some areas (4 and 40) reaches its adult pattern only after 11 years. Considerable modifications occur during development in the relative activities of the different cortical layers. SDH activity initially appears in layers which send projections to subcortical relays or receive specific thalamocortical afferents. Layers which receive cortico-cortical projections mature later on. In contrast to SDH activity that of M alpha GPDH is elevated already before birth, increases until one month of age and decreases afterwards. This enzyme present in neuroblasts disappears from mature cortical neurons, except from those of the VIth layer. The distribution of this enzyme in cortical layers of the child is opposite to that of SDH. The developmental evolution of these two enzymes has been confirmed by biochemical assays.