Microtubules in Plasmodium falciparum merozoites and their importance for invasion of erythrocytes
Microtubules in Plasmodium falciparum merozoites and their importance for invasion of erythrocytes
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DOI：
10.1017/s003118209800328x
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发表时间：
1998-11-01
期刊：
影响因子：
2.4
通讯作者：
Mitchell, GH
中科院分区：
文献类型：
--
作者：
Fowler, RE;Fookes, RE;Mitchell, GH
恶性疟原虫裂殖子具有2-3个膜下微管的阵列，称为f-MBST。我们以前已经表明，秋水仙素抑制裂殖子侵入红细胞，表明在这个过程中的微管参与。在秋水仙素暴露之前，裂殖子微管的Taxol（R）稳定作用降低了秋水仙素对侵袭的抑制作用。免疫荧光分析表明，f-MASTs的数量和长度减少，在秋水仙素处理的裂殖子，确认微管是秋水仙素抑制的目标。二硝基苯胺类药物，氟乐灵和二甲戊乐灵，通过免疫荧光法显示，以determining f-MAST和两种药物在侵袭试验中抑制。这些结果表明，f-MAST的完整性对于成功入侵是重要的。荧光成像显示线粒体与f-MAST的对齐，表明线粒体运输可能在具有无序f-MAST的裂殖子中受到干扰。在晚期裂殖子中解聚mt并不影响线粒体向裂殖子的分配。
Plasmodium falciparum merozoites have an array of 2-3 subpellicular microtubules, designated f-MBST. We have previously shown that colchicine inhibits merozoite invasion of erythrocytes, indicating a microtubular involvement in this process. Colchicine inhibition of invasion was reduced by the Taxol(R)-stabilization of merozoite microtubules prior to colchicine exposure. Immunofluorescence assays showed that the number and length of f-MASTs were reduced in colchicine-treated merozoites, confirming that microtubules were the target of colchicine inhibition. The dinitroaniline drugs, trifluralin and pendimethalin, were shown by immunofluorescence to depolymerize the f-MAST and both drugs were inhibitory in invasion assays. These results demonstrate that the integrity of the f-MAST is important for successful invasion. Fluorescence imaging demonstrated the alignment of mitochondria to f-MAST, suggesting that mitochondrial transport might be perturbed in merozoites with disorganized f-MAST. Depolymerizing mt in late-stage schizonts did not affect the allocation of mitochondria to merozoites.