Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells
Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells
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鼻咽癌细胞放射抗性相关蛋白热休克蛋白27的鉴定
DOI：
10.1007/s00432-012-1293-0
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
Zhang B, Qu JQ, Xiao L, Yi H, Zhang PF, et al.
中科院分区：
文献类型：
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作者：
Zhang B, Qu JQ, Xiao L, Yi H, Zhang PF, et al.
目的鉴定鼻咽癌(NPC)细胞中参与放射抗性的蛋白。方法采用亚致死电离辐射，从其亲代鼻咽癌细胞系CNE1中建立抗辐射鼻咽癌细胞系。采用克隆形成存活测定、细胞生长测定和流式细胞术分析来检查抗辐射CNE1细胞（CNE1-IR）和对照CNE1细胞的放射敏感性差异。进行比较蛋白质组学以鉴定两种细胞系中的差异蛋白质。选择 CNE1-IR 细胞中上调蛋白之一的 HSP27 与鼻咽癌细胞放射抗性之间的关联，使用反义寡核苷酸 (ASO)、克隆存活实验、凋亡细胞的 Hoechst 33258 染色和细胞活力的 MTT 检测进行进一步研究。 结果建立了源自其亲本细胞系 CNE1 的放射抗性鼻咽癌细胞系 CNE1-IR。通过蛋白质组学鉴定了CNE1-IR和CNE1细胞中的13种差异蛋白，并通过蛋白质印迹选择性地证实了其中一种蛋白HSP27的差异表达。 HSP27 ASOs抑制HSP27的表达，降低了CNE1-IR细胞在辐射后的克隆存活率和细胞活力，增加了细胞凋亡，即增强了CNE1-IR细胞的放射敏感性。结论数据提示，HSP27是鼻咽癌细胞中的一种放射抗性蛋白，其上调可能与鼻咽癌的放射抗性有关。
PurposeTo identify the proteins involved in radioresistance in nasopharyngeal cancer (NPC) cells.MethodsSublethal ionizing radiation was applied to establish a radioresistant NPC cell line from its parental NPC cell line CNE1. Clonogenic survival assay, cell growth assay and flow cytometry analysis were used to examine the difference of radiosensitivity in the radioresistant CNE1 cells (CNE1-IR) and control CNE1 cells. Comparative proteomics was performed to identify the differential proteins in the two cell lines. Association of HSP27, one of upregulated proteins in CNE1-IR cells, with NPC cell radioresistance was selected for further investigation using antisense oligonucleotides (ASOs), clonogenic survival assay, Hoechst 33258 staining of apoptotic cells and MTT assay of cell viability.ResultsRadioresistant NPC cell line CNE1-IR derived from its parental cell line CNE1 was established. Thirteen differential proteins in the CNE1-IR and CNE1 cells were identified by proteomics, and differential expression of HSP27, one of identified proteins, was selectively confirmed by western blot. Inhibition of HSP27 expression by HSP27 ASOs decreased clonogenic survival and cell viability and increased cell apoptosis of CNE1-IR cells after irradiation, that is, enhanced radiosensitivity of CNE1-IR cells.ConclusionThe data suggest that HSP27 is a radioresistant protein in NPC cells, and its upregulation may be involved in the NPC radioresistance.
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DOI：
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发表时间：
2010
期刊：
--
影响因子：
--
作者：
Xue-Ping Feng;Hong Yi;Maoyu Li;Xin-Hui Li;B. Yi;Pengfei Zhang;Cui Li;Fang Peng;Can‐E. Tang-Can‐E.
通讯作者：
Xue-Ping Feng;Hong Yi;Maoyu Li;Xin-Hui Li;B. Yi;Pengfei Zhang;Cui Li;Fang Peng;Can‐E. Tang-Can‐E.
影响因子：
3
作者：
Shihong Yang;J Wu;Y. Zuo;L. Tan;H. Jia;H. Yan;X. Zhu;M. Zeng;J. Ma;W. Huang
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Shihong Yang;J Wu;Y. Zuo;L. Tan;H. Jia;H. Yan;X. Zhu;M. Zeng;J. Ma;W. Huang
影响因子：
3.7
作者：
Ma X;Yang L;Xiao L;Tang M;Liu L;Li Z;Deng M;Sun L;Cao Y
通讯作者：
Cao Y
DOI：
10.1016/s1011-1344(96)00010-3
发表时间：
1997-05
期刊：
Journal of photochemistry and photobiology. B, Biology
影响因子：
--
作者：
F. Trautinger;C. Kokesch;I. Herbacek;R. Knobler;I. Kindås-mügge
通讯作者：
F. Trautinger;C. Kokesch;I. Herbacek;R. Knobler;I. Kindås-mügge
DOI：
10.1016/b978-0-12-814936-2.00002-x
发表时间：
2019
期刊：
Nasopharyngeal Carcinoma
影响因子：
--
作者：
M. Lung;Wei Dai;J. Ko
通讯作者：
M. Lung;Wei Dai;J. Ko