Induction of apoptosis by green tea catechins in human prostate cancer DU145 cells
Induction of apoptosis by green tea catechins in human prostate cancer DU145 cells
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DOI：
10.1016/s0024-3205(00)01020-1
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发表时间：
2001-01-26
期刊：
影响因子：
6.1
通讯作者：
Kwok, TT
中科院分区：
文献类型：
--
作者：
Chung, LY;Cheung, TC;Kwok, TT
绿色茶儿茶素（GTC）包括（-）-表没食子儿茶素-3-没食子酸酯（EGCG）、（-）-表没食子儿茶素（EGC）、（-）-表儿茶素-3-没食子酸酯（ECG）和（-）-表儿茶素（EC），它们除了具有化学预防作用外，还显示出在多种细胞系统中抑制细胞生长和诱导细胞凋亡。本研究发现，除EC无活性外，绿色茶提取物（TE）等3种GTC均能抑制人前列腺癌DU 145细胞的生长，诱导细胞凋亡，其机制主要是通过增加活性氧的生成和线粒体去极化。结果表明，不同GTC在生长抑制、凋亡诱导、ROS生成和线粒体去极化方面的作用顺序相似，即ECG > EGCG > EGC > EC。尽管其分子机制尚不清楚，但GTC诱导的细胞凋亡与BCL-2家族成员无关，因为表没食子儿茶素没食子酸酯（EGCG）没有改变DU 145细胞中BCL-2、BCL-X（L）和BAD的表达。(C)2001 Elsevier Science Inc. All rights reserved.
Green tea catechins (GTCs) including (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (ECG) and (-)-epicatechin (EC) were shown to suppress cell growth and induce apoptosis in various cell systems in addition to their chemo-preventive effect. In this study, except EC which was inactive, green tea extract (TE) and other 3 GTCs were found to suppress the growth and induce apoptosis in human prostate cancer DU145 cells largely through an increase in reactive oxygen species formation and mitochondrial depolarization. The conclusion was supported by the fact that the profiles for different GTCs in growth suppression, apoptosis induction, ROS formation and mitochondrial depolarization are in a similar order, i.e. ECG > EGCG > EGC > EC. Although the molecular mechanisms are still not clear, apoptosis induced by GTCs is not related to the members of BCL-2 family as EGCG did not alter the expression of BCL-2, BCL-X(L) and BAD in DU145 cells. (C) 2001 Elsevier Science Inc. All rights reserved.