COMPARISON OF 3 METHODS FOR THE DETERMINATION OF OXYSTEROLS IN SPRAY-DRIED EGG
COMPARISON OF 3 METHODS FOR THE DETERMINATION OF OXYSTEROLS IN SPRAY-DRIED EGG
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DOI：
10.1016/0021-9673(95)00034-k
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发表时间：
1995-06-30
影响因子：
4.1
通讯作者：
BOATELLA, J
中科院分区：
文献类型：
--
作者：
GUARDIOLA, F;CODONY, R;BOATELLA, J
比较了联合收割机不同纯化步骤的气相色谱法测定喷雾干燥鸡蛋中氧化甾醇的方法。此外，使用四种不同的洗脱系统，随着极性的增加，二氧化硅柱在OS纯化中的效率进行了研究。检查分析方法应用期间是否存在胆固醇氧化，并确定响应线性以及色谱检测限和定量限。通过计算不同操作系统的精密度和回收率对方法进行了表征。单独基于皂化的方法被拒绝，因为它显示出低得多的精确度。包括皂化和硅胶柱纯化的方法比单独基于柱纯化的方法提供更高的可靠性。因为它显示了更高的精度，并且可以处理更大的样品，这提高了检测和定量的限度。
Three methods for the GC determination of oxysterols (OSs) in spray-dried egg, which combine different steps of purification, are compared. In addition, the efficiency of silica cartridges in the purification of OSs using four different systems of elution with increasing polarities is studied. The absence of cholesterol oxidation during the application of the analytical procedures is checked, and the linearity of the response and the chromatographic limits of detection and quantification are established. The methods are characterized by the calculation of precision and recovery for the different OSs. The method based on saponification alone is rejected, since it shows much lower precision. The method that includes saponification and silica cartridge purification offers higher reliability than the method based on cartridge purification alone? because it shows a higher precision and larger samples can be processed, which improves the limits of detection and quantification.