Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis
Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis
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DOI：
10.1007/s10535-012-0007-4
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发表时间：
2012
影响因子：
1.5
通讯作者：
B. Zhou;N. Li;Z. Zhang;X. Huang;H. Chen;Z. Hu;X. Pang;W. Liu;Y. Lu
中科院分区：
文献类型：
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作者：
B. Zhou;N. Li;Z. Zhang;X. Huang;H. Chen;Z. Hu;X. Pang;W. Liu;Y. Lu
荔枝幼穗发育过程中营养生长与生殖生长存在着强烈的竞争关系。本文研究了过氧化氢和一氧化氮在幼叶生长和穗发育竞争中的作用。结果表明，低温诱导开花增加了混合芽中H2 O2和NO的含量。用NO供体硝普钠（SNP）和超氧阴离子产生剂甲基紫精（MV）处理混合芽，NO和H2 O2含量增加。MV和SNP处理促进了幼叶的脱落，并促进了幼穗的发育。一氧化氮合酶抑制剂N ω-硝基-L-精氨酸甲酯（L-NAME）和过氧化氢捕获剂二甲基硫脲（DMTU）抑制低温诱导的开花。SNP促进荔枝LEAFY同源基因（LcLFY）的表达。NO清除剂2-苯基-4，4，5，5-四甲基咪唑啉-1-氧化物（PTIO）和H_2O_2捕捉剂DMTU抑制了上述促进作用。结果表明，H2 O2和NO通过抑制未分化叶的生长和促进花相关基因LcLFY的表达而促进生殖生长。
Vegetative growth and reproductive growth strongly competes with each other during panicle development in litchi (Litchi chinensisSonn.). We herein investigated the roles of hydrogen peroxide and nitric oxide in the competition between growth of rudimentary leaves and panicle development. The results show that the chilling-induced flowering increased H2O2and NO contents in the mixed buds. Treatments with sodium nitroprusside (SNP), the NO donor, and methyl viologen dichloride hydrate (MV), the superoxide generator, increased NO and H2O2contents in the mixed buds. MV and SNP treatments promoted abscission of rudimentary leaves and encouraged panicle development before or at the stage of panicle emergence. The nitric oxide synthase inhibitorNω-nitro-L-arginine methyl ester (L-NAME) and the H2O2trapper dimethylthiourea (DMTU) inhibited a chilling-induced flowering. SNP promoted the expression of litchiLEAFYhomolog (LcLFY). These promotive effects were suppressed by the NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl3-oxide (PTIO) and the H2O2trapper, DMTU. The results suggest that H2O2and NO promote reproductive growth by inhibiting the growth of rudimentary leaves as well as by promoting the expression of the flower related gene,LcLFY.