Paladin is an antiphosphatase that regulates neural crest cell formation and migration.
Paladin is an antiphosphatase that regulates neural crest cell formation and migration.
复制标题
DOI：
10.1016/j.ydbio.2012.08.007
复制
发表时间：
2012-11-15
影响因子：
2.7
通讯作者：
Gammill LS
中科院分区：
文献类型：
--
作者：
Roffers-Agarwal J;Hutt KJ;Gammill LS
Although a network of transcription factors that specifies neural crest identity in the ectoderm has been defined, expression of neural crest transcription factors does not guarantee eventual migration as a neural crest cell. While much work has gone into determining regulatory relationships within the transcription factor network, the ability of protein modifications like phosphorylation to modulate the function of neural crest regulatory factors and determine when and where they are active also has crucial implications. Paladin, which was previously classified as a phosphatase based on sequence similarity, is expressed in chick neural crest precursors and is maintained throughout their epithelial to mesenchymal transition and migration. Loss of Paladin delays the expression of transcription factors Snail2 and Sox10 in premigratory neural crest cells, but does not affect accumulation of FoxD3, Cad6B or RhoB, indicating that Paladin differentially modulates the expression of genes previously thought to be coregulated within the neural crest gene regulatory network. Both gain and loss of Paladin function result in disrupted neural crest migration, reinforcing the importance of precisely regulated phosphorylation for neural crest migration. Mutation of critical, catalytic cysteine residues within Paladin’s predicted phosphatase active site motifs did not abolish the function of Paladin in the neural crest. Collectively, these data indicate that Paladin is an antiphosphatase that modulates the activity of specific neural crest regulatory factors during neural crest development. Our work identifies a novel regulator of phosphorylation status that provides an additional layer of regulation in the neural crest.
登录
查看更多内容
影响因子：
64.8
作者：
Basch, ML;Bronner-Fraser, M;García-Castro, MI
通讯作者：
García-Castro, MI
影响因子：
14.8
作者：
Heckman, Karin L.;Pease, Larry R.
通讯作者：
Pease, Larry R.
DOI：
10.1146/annurev.cellbio.042308.113245
发表时间：
2010
影响因子：
11.3
作者：
Betancur P;Bronner-Fraser M;Sauka-Spengler T
通讯作者：
Sauka-Spengler T
影响因子：
3.7
作者：
Huang SM;Hancock MK;Pitman JL;Orth AP;Gekakis N
通讯作者：
Gekakis N
影响因子：
4.6
作者：
Banerjee, Santanu;Gordon, Laura;Granato, Michael
通讯作者：
Granato, Michael