Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
复制标题
猪YL氨基酸转运蛋白1的分子克隆、组织分布及表达
DOI：
10.5713/ajas.2010.90275
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
冯定远
中科院分区：
文献类型：
--
作者：
冯定远
In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y + LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine y + LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat y + LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest y + LAT1 mRNA abundance and the lung had the lowest y + LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine y + LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
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DOI：
10.1007/s004240000274
发表时间：
2000-08
期刊：
Pflügers Archiv
影响因子：
--
作者：
F. Verrey;C. Meier;G. Rossier;L. Kühn
通讯作者：
F. Verrey;C. Meier;G. Rossier;L. Kühn
DOI：
10.1007/s002329900595
发表时间：
1999-12
期刊：
The Journal of Membrane Biology
影响因子：
--
作者：
F. Verrey;Donald L. Jack;I. Paulsen;M. Saier;R. Pfeiffer
通讯作者：
F. Verrey;Donald L. Jack;I. Paulsen;M. Saier;R. Pfeiffer
影响因子：
5.3
作者：
Keith M. Gottesdiener;Beverly A. Karpinski;T. Lindsten;L. Jack;Strominger;Nancy H. Jones;'. C. B. Thompson;Jeffrey M. Leiden
通讯作者：
Keith M. Gottesdiener;Beverly A. Karpinski;T. Lindsten;L. Jack;Strominger;Nancy H. Jones;'. C. B. Thompson;Jeffrey M. Leiden
影响因子：
64.8
作者：
Mastroberardino, L;Spindler, B;Verrey, F
通讯作者：
Verrey, F
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen