Stability of mammalian lens phosphofructokinase.
Stability of mammalian lens phosphofructokinase.
复制标题
哺乳动物晶状体磷酸果糖激酶的稳定性。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
E. Barañano
中科院分区：
文献类型：
--
作者：
H. Cheng;L. Chylack;J. Chien;E. Barañano
Two interconvertible phosphofructokinase (PFK) forms were found in rat and human lenses; whereas only one predominant form was found in calf lens. PFK isolated from these lenses possessed a common property, i.e., pH-dependent cold (or acid) lability. The inactivation was prevented by including either adenosine triphosphate (ATP) or fructose-6-phosphate (fru-6-P) in the incubating media. The protective effect of ATP or fru-6-P was complete in rat or calf lenses. In human lens, although fru-6-P was fully protective, ATP protected only partially. The inactivation could be reversed by addition of fru-6-P, but not ATP, to the incubating media at 37.5 degrees C. Lens organ culture studies showed that the depletion of lenticular ATP seemed to precipitate the loss of PFK.
